Abstract
The chemical shifts of the protons in the proflavine and acridine orange molecules in aqueous solution were measured by 1H NMR spectroscopy. The equilibrium constants for the association of the molecules and the chemical shifts of the protons in the monomers and associates were obtained from the concentration dependence of the proton chemical shifts. The most probable structures for the dimers of the dyes were calculated on the basis of the obtained chemical shifts, and a comparative analysis was made.
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Literature cited
D. J. Blears and S. S. Danyluk, “A nuclear magnetic resonance investigation of the aggregation of acridine orange in aqueous solution,” J. Am. Chem. Soc., 89, No. 1, 21–26 (1967).
M. P. Heyn and R. Bretz, “The self-association of ATP; thermodynamics and geometry,” Biophys. Chem., 3, 34–45 (1975).
É. L. Kulikov, A. N. Veselkov and L. N. Dymant, “Application of the associative variation principle for the treatment of data from a physical experiment,” Ukr. Fiz. Zh., 29, No. 10, 1477–1484 (1984).
A. N. Veselkov, L. N. Dymant, and É. L. Kulikov, “Application of variation methods of treatment of experimental data during investigation of the aggregation of the molecules of acridine dyes by high-resolution nuclear magnetic resonance,” Khim. Fiz., 3, No. 8, 1101–1108 (1984).
Software for the ES computer. Unconditional minimization of functions [in Russian], Vol. 17, Part 10, Minsk (1981).
R. I. Abraham, The Application of Aromatic Ring Currents in the Elucidation of Drug-Ligand and Metallo-porphyrin Complexations. Nuclear Magnetic Resonance Spectroscopy in Molecular Biology, Reidel, Dordrecht (1978), pp. 461–479.
A. N. Veselkov, L. N. Dymant, and S. F. Baranovskii, “Investigation of the reaction of proflavine with adenosine monophosphate in aqueous solution by proton magnetic resonance,” Khim. Fiz. 5, No. 10, 1334–1339 (1986).
A. N. Veselkov and L. N. Dymant, “Investigation of the structures of complexes of proflavine with dinucleotides in aqueous solution by 1H NMR,” Zh. Strukt. Khim., 27, No. 6, 73–78 (1986).
A. Delbarre, B. P. Roques, J. B. LePecq, et al., “PMR studies of the self-association of DNA intercalating ellipticine derivatives in aqueous solution,” Biophys. Chem., 4, 275–279 (1976).
T. R. Krugh and Yu. Chih Chen, “Actinomycin D deoxynucleotide complexes as models for the actinomycin D-DNA complex. The use of nuclear magnetic resonance to determine the stoichiometry and the geometry of the complexes,” Biochemistry, 14, No. 22, 4912–4922 (1975).
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Translated from Teoreticheskaya i Éksperimental'naya Khimiya, Vol. 23, No. 3, pp. 373–376, May–June, 1987.
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Veselkov, A.N., Dymant, L.N. & Dreval', S.S. Investigation of the self-association of proflavine and acridine orange molecules in aqueous solution by 1H NMR. Theor Exp Chem 23, 346–350 (1987). https://doi.org/10.1007/BF00531393
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DOI: https://doi.org/10.1007/BF00531393