Summary
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1.
The effect of added homogenates of liver-, spleen-, kidney-, and lung tissue from normal as well as tumour-bearing rats was studied on the incorporation of labelled (S35) methionine into the proteins of Ehrlich ascites carcinoma in vitro. In some instances rat liver-homogenate was used instead of the Ehrlich tumour.
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2.
All preparations used showed a pronounced effect on the methionine incorporation. This was strictly dose-dependent, some doses inducing stimulation while others inhibited this process.
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3.
No basic differences were found in the effect of the same tissue from normal-and tumour-bearing animals, nor from different tissues from the same animal, respectively. Nevertheless, some differences were encountered, caused presumably by different amounts of the active substance being present in various tissues.
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4.
Since the results of these experiments conform strictly with those of the effect of carcinogenic hydrocarbons on the incorporation of labelled amino acids, an assumption has been made that a carcinogenic substance may be present in tissues capable of influencing their growth by acting on the biosynthesis of proteins.
Zusammenfassung
Es wurde die Wirkung von Leber-, Milz-, Nieren- und Lungenhomogenaten von normalen und tumortragenden Ratten auf den Einbau von S35-markiertem Methionin in die Proteine des Ehrlich-Ascitescarcinoms untersucht; in einigen Fällen wurde anstelle des Ehrlich-Ascitestumors Rattenleberhomogenat benutzt.
Alle verwendeten Präparationen zeigten eine deutliche Beeinflussung der Einbaurate des Methionins, die sich in Abhängigkeit von der Menge der zugesetzten Gewebshomogenate teils im Sinne einer Stimulierung, teils im Sinne einer Hemmung auswirkte. Dabei bestanden zwischen Homogenaten der verschiedenen Organe von normalen und tumortragenden Tieren keine grund-legenden Unterschiede der Wirkung. Soweit Unterschiede vorhanden waren, werden sie auf den unterschiedlichen Gehalt der einzelnen Gewebe an der für die Wirkung verantwortlichen aktiven Substanz zurückgeführt.
Auf die Gleichartigkeit dieser Befunde mit Ergebnissen über die Wirkung von cancerogenen Kohlenwasserstoffen auf den Einbau von markierten Aminosäuren wird hingewiesen, wobei angenommen wird, daß eine (endogene) cancerogene Substanz in Geweben vorhanden sei, welche deren Wachstum über eine Einwirkung auf die Biosynthese von Proteinen beeinflußt.
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Hradec, J. Effect of added tissue-homogenates on the incorporation of labelled methionine into the proteins of Ehrlich ascites carcinoma in vitro. Z Krebs-forsch 63, 4–11 (1959). https://doi.org/10.1007/BF00531344
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DOI: https://doi.org/10.1007/BF00531344