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Bestimmung von Xanthinoxidase in Gewebekulturen, Gewebeproben und Tumor-Zellsuspensionen

Determination of xanthine oxidase in tissue cultures, in tissue slices and in tumor-cell suspensions

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Summary

After incubation of the biological material with highly labelled hypoxanthine-8-14C and chromatography of the medium, the radioscans gave a survey of the metabolites formed. Xanthine oxidase activity was calculated after integration of the individual peaks of radioactivity. In contrast to the statements of various authors, our results gave no indication of a reduced activity of xanthine oxidase as a common quality of all tumor tissues (e. g., Walker cells and rat fibroblasts had the same xanthine oxidase activity: HeLa cells showed little activity, and human fibroblasts none). The xanthine oxidase contents of cells show more variation among various normal tissues than between normal tissues and tumor tissue.

Zusammenfassung

Nach Inkubation des biologischen Materials mit hochmarkiertem Hypoxanthin-8-14C ergab die Chromatographie des Mediums aus der Verteilung der Radioaktivität einen Überblick über die gebildeten Metaboliten. Die Xanthinoxidase-Aktivität wurde nach Integration der einzelnen Gipfel der Radioaktivität berechnet. Im Gegensatz zu Angaen verschiedener Autoren zeigen unsere Resultate keinen Anhalt für eine verminderte Xanthinoxidase-Aktivität als eine allgemeine Eigenschaft von Tumorgewebe (z. B. hatten Walker-Zellen und Ratten-Fibroblasten die gleiche Aktivität; HeLa-Zellen zeigten geringe und menschliche Fibroblasten keine Xanthinoxidase-Aktivität). Der Gehalt an Xanthin-oxidase-Aktivität zeigt größere Variationen zwischen verschiedenen normalen Geweben als zwischen normalen und malignen Geweben.

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Literatur

  • Bergel, F., Bray, R.C., Haddow, A., Levin, I.: Enzymic control of purines by xanthine oxidase. In: Chemistry and Biology of Purines (Ciba Foundation Symposion), pp. 256–269. London: Churchill 1957.

    Google Scholar 

  • Bergel, F., Everett, J. L., Martin, J. B., Webb, J. S.: Cellular constituents. Major and minor metals in normal and abnormal tissues. J. Pharm. Pharmacol. 9, 522–531 (1957).

    Google Scholar 

  • Burch, H. B., Lowry, O. H., Padilla, E. J.: Effects of riboflavin in deficiency and realimentation of flavin enzymes of tissues. J. biol. Chem. 223, 29–37 (1956).

    Google Scholar 

  • DeLamirande, G., Allard, C., Cantaro, A.: Purine-metabolizing enzymes in normal rat liver Nandovikoff hepatoma. Cancer Res. 18, 952–958 (1958).

    Google Scholar 

  • Dietrich, L. S., Shapiro, D. M.: Combination chemotherapy of cancer: Potentiation of carcinostatic action of 8-Azaguanine by a riboflavin analog. Cancer Res. 13, 699–701 (1953).

    Google Scholar 

  • Feigelson, Ph., Ultmann, J. E., Harris, S., Dashman, Th.: Cellular xanthine oxidase and uricase levels in leukemic and normal mouse leukocytes. Cancer Res. 10, 1230–1233 (1959).

    Google Scholar 

  • Haining, J. L., Legan, J. S.: Fluorometric assay for xanthine oxidase. Anal. Biochem. 21, 337–343 (1967).

    Google Scholar 

  • Kalckar, H. M.: Differential spectrometry of purine compounds by means of specific enzymes. J. biol. Chem. 167, 429–435 (1947).

    Google Scholar 

  • Lettré, H.: Cytotoxic agents of the purine and sterol group. In: Progress in experimental Tumor Research, Vol. 1, pp. 329–359. Basel: Karger 1960.

    Google Scholar 

  • —, Immich, H., Marquetand, D., Werner, D.: Stimulierung der Proteinsynthese durch cytotoxisches Puryl-6-tryptamin. Naturwissenschaften 56, 517 (1969).

    Google Scholar 

  • Lowry, O. H.: In: Methods in Enzymology, Vol. 4, p. 380 (Hrsg. S. P. Colowick und N. O. Kaplan). New York: Academic Press 1957.

    Google Scholar 

  • Scholz, W., Paweletz, N.: Glykogenablagerung in Zellkernen des Ehrlich-Ascites-Tumors. Z. Krebsforsch. 72, 211–212 (1969).

    Google Scholar 

  • Watts, R. W. E., Watts, J. E., Seegmiller, J. E.: Xanthine oxidase activity in human tissues and its inhibition by allopurinol (4-hydroxypyrazolo[3,4-D]pyrimidine). J. Lab. clin. Med. 66, 688–697 (1965).

    Google Scholar 

  • Werner, D.: Enzymatischer Abbau xenobiotischer Purinderivate. In: Aktuelle Probleme aus dem Gebiet der Cancerologie II (Zweites Heidelberger Symposion, hrsg. v. H. Lettré u. G. Wagner), S. 235–238. Berlin-Heidelberg-New York: Springer 1968.

    Google Scholar 

  • — Schleich, A.: Biochemische Vorgänge bei der Behandlung von Zellen mit Puryl-6-histamin. Mat. Med. Nordmark 20, 613–618 (1968).

    Google Scholar 

  • Westerfeld, W. W., Richert D., Hilfinger, M. F.: Studies on xanthine oxidase during carcinogenesis by p-Dimethylaminozobenzene. Cancer Res. 10, 486–494 (1960).

    Google Scholar 

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Authors and Affiliations

  1. Institut für experimentelle Krebsforschung am Deutschen Krebsforschungszentrum, Heidelberg

    H. Lettré, D. Werner & A. Schleich

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  1. H. Lettré
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  2. D. Werner
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  3. A. Schleich
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Herrn Prof. Dr. K. H. Bauer zu seinem 80. Geburtstag gewidmet.

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Lettré, H., Werner, D. & Schleich, A. Bestimmung von Xanthinoxidase in Gewebekulturen, Gewebeproben und Tumor-Zellsuspensionen. Z Krebs-forsch 74, 368–375 (1970). https://doi.org/10.1007/BF00531219

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  • DOI: https://doi.org/10.1007/BF00531219

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