Zusammenfassung
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1.
Acetoxy-, N-Hydroxy-und Acetamidofluoren reduzieren Thymidin-, Uridin- und Leucineinbau in HeLa und Hamsterembryo-Zellen in der angegebenen Reihenfolge, die auch ihrer carcinogenen Aktivität entspricht.
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2.
“DNA- und RNA-Synthese” ist stärker reduziert als die “Proteinsynthese”.
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3.
Tetrahymena Pyriformis spiegelt die carcinogene Aktivität nicht wieder.
Summary
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(1)
Acetoxy-, N-hydroxy- and acetamidofluorene reduce “DNA, RNA and protein synthesis” as measured by the incorporation of tritium labelled precursors into HeLa and hamster embryo cells in the order given, thus reflecting their carcinogenic activities.
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(2)
“DNA and RNA synthesis” is more dramatically affected than “protein synthesis”.
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(3)
Tetrahymena pyriformis does not reflect the carcinogenic activities of the tested compounds.
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References
Berwald, Y., Sachs, L.: In vitro transformation of normal cells to tumor cells by carcinogenic hydrocarbons. J. nat. Cancer Inst. 35, 641–661 (1965).
Diamond, L.: The interaction of chemical carcinogens and cells in vitro. Progr. Exp. Tumor Res. 11, 364–383 (1969).
Huebner, G., R. Ludwig: Zur Permeationsbeeinflussung durch Dimethylsulfoxid. Naturwissenschaften 56, 221 (1969).
Miller, J. A., E. C. Miller: The metabolic activation of carcinogenic aromatic amines and amides Progr. Exp. Tumor Res. 11, 273–301 (1969).
Mondal, S., Heidelberger, C.: In vitro malignant transformation by methylcholanthrene of the progeny of sinle cells derived from C3H mouse prostate. Proc. nat. Acad. sci. (Wash.) 65, 219–225 (1970).
Süss, R., M. Volm: Rapid screening of macromolecular synthesis in single cell cultures. Naturwissenschften 55, 134 (1968).
Volm, M., R. Süss: Einbau von 3H-Thymidin, 3H-Uridin und 3H-Leucin in Tetrahymena, L-Zellen und Leberexplantate unter Einfluß von carcinogenen Nitrosamiden. Naunyn-Schmiedebergs Arch. Pharmak. u. exp. Path. 261, 353–359 (1968).
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Herrn Prof. Dr. Dr. h. c. Dr. h. c. Dr. h. c. K. H. Bauer zur Vollendung des 80. Lebensjahres zugeeignet.
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Süss, R., Kinzel, V., Volm, M. et al. Effect of acetamidofluorene and activated derivatives on “Macromolecular synthesis” in single cell cultures. Z Krebs-forsch 74, 338–343 (1970). https://doi.org/10.1007/BF00531215
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DOI: https://doi.org/10.1007/BF00531215