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Automatic methods for the determination of total dissolved and particulate carbohydrates in the marine environment

Automatische Methoden zur Bestimmung von gelöstem und partikulärem Gesamt-Kohlenhydrat in Meerwasser

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  • Aquatic Environment
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Zusammenfassung

Die für die Bestimmung gelösten und partikulären Gesamt-Kohlenhydrats in Seewasser empfohlenen Methoden basieren beide auf der l-TryptophanSchwefelsäure-Reaktion. Die Messungen erfolgen automatisch im Technicon AutoAnalyzer II. Zur Eichung wird Glucose benutzt. Störendes Nitrat und Nitrit werden ebenfalls mit einem Technicon AutoAnalyzer II gemessen; die entsprechenden Werte werden nach Eichung vom Gesamtsignal des Zuckeranalysators abgezogen. Partikuläre Kohlenhydrate werden nach Aufschluß des Rückstandes auf Glasfaserfiltern mit Schwefelsäure gemessen. Beide Methoden liefern genaue Ergebnisse im Bereich von 0.1–30 μmol/l.

Summary

The methods recommended for the determination of total dissolved and particulate carbohydrates in the marine environment are based on the l-tryptophan/sulfuric acid reaction. Automatic measurements are performed with a Technicon AutoAnalyzer II. Glucose is used for the calibration procedure. Interfering nitrate and nitrite are also measured with a Technicon AutoAnalyzer II; the values are subtracted from the total reading of the carbohydrate analyzer after calibration. Particulate carbohydrates are measured after sulfuric acid digestion of the residue on glassfiber filters. Both methods permit total carbohydrates to be determined exactly in the concentration range from 0.1–30 μmol/l.

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Authors and Affiliations

  1. Institut für Biochemie und Lebensmittelchemie, Universität Hamburg, Martin-Luther-King-Platz 6, D-2000, Hamburg 13, Bundesrepublik Deutschland

    K. Eberlein & M. Schütt

Authors
  1. K. Eberlein
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  2. M. Schütt
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Eberlein, K., Schütt, M. Automatic methods for the determination of total dissolved and particulate carbohydrates in the marine environment. Z. Anal. Chem. 323, 47–49 (1986). https://doi.org/10.1007/BF00531130

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  • DOI: https://doi.org/10.1007/BF00531130

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