Advertisement

Zeitschrift für Krebsforschung

, Volume 62, Issue 4, pp 361–369 | Cite as

Über die Veränderungen der Nucleinsäuren in der Leber von Ratten bei der Cancerogenese durch 4-Dimethylaminoazobenzol

  • A. A. Hadjiolov
  • Z. S. Tentschewa
Article

Summary

By histochemical (Brachet) and biochemical (Ogur and Rosen) method the nucleic acids in the rat liver during experimental cancerogenesis by 4-Dimethylaminoazobenzene were studied.

In the first period of cancerogenesis (45–50 days) hepatic RNA decreases, while DNA increases. The low level of RNA is a consequence of its disappearence in the dying hepatic cells and of the poverty of RNA in the rapidly growing cholangioepithelial cells.

In the second period of cancerogenesis (95–100 days) the content of RNA begins to increase together with the amount of DNA. The increasing of RNA is correlated with large areas of cholangiofibrosis in which the chnloagioepithelial cells are rich in cytoplasmic RNA.

Tumors with “hepatoma” or “cholangioma” structure are characterised by a high level of RNA, the same as in normal liver when expressed per fresh weigth and even higher than in normal liver when expressed per dry weigth. The DNA content of tumors is also very high. Histochemically the cytoplasm and the nucleolar apparatus of tumor cells are rich in RNA.

The changes in adjacent to the tumors parenchyma are similar to that characteristic for the first and the second period of cancerogenesis.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. 1.
    Beloussova, A. K.: Veränderungen in der intracellulären Verteilung von Nucleinsäuren während des Malignisationsprozesses. Dokl. Akad. Nauk SSSR, 97, 157 (1954).Google Scholar
  2. 2.
    Butenandt, A., u. H. Dannenberg: Die Biochemie der Geschwülste. In: Handbuch der allgemeinen Pathologie, Bd. 6, Teil III, S. 107. Berlin: Springer 1956.Google Scholar
  3. 3.
    Cantero, A., R. Daoust and G. de Lamirande: Nucleodepolymerase Activity of Precancerous Rat Liver. Science 112, 221 (1950).Google Scholar
  4. 4.
    Cunningham, L., A. Griffin and J. M. Luck: Effect of a Carcinogenic Azo Dye on Liver Cell Structure. Isolation of Nuclei and Cytoplasmic Granules. Cancer Res. 10, 194 (1950).Google Scholar
  5. 5.
    Davidson, J. N., and R. M. S. Smellie: Phosphorus Compounds in the Cell. III. The Incorporation of Radioactive Phosphorus into the Ribonucleotide Fraction of Liver tissue. Biochem. J. 52, 599 (1952).Google Scholar
  6. 6.
    Griffin, A., W. N. Nye, L. Noda and J. M. Luck: Tissue Proteins and Carcinogenesis. I. The Effect of Carcinogenic Azo Dyes on Liver Proteins. J. biol. Chem. 176, 1225 (1948).Google Scholar
  7. 7.
    Hadjiolov, A. A.: Experimental Cancer of the Liver Induced by 4-Dimethylaminoazobenzene. I. The Dietary Regimen and the Induction of Cancer. The Role of Vitamin A. (Bulgarisch, res. Russ., Engl.) Bull. Inst. Morph. Acad. Sci. Bulgarie 2, 423 (1957).Google Scholar
  8. 8.
    Hadjiolov, A. A.: Experimental Cancer of the Liver Induced by 4-Dimethylaminoazobenzene. II. Histological Changes in the Liver During the Cancerogenesis. (Bulgarisch, res. Russ., Engl.) Bull. Inst. Morph. Acad. Sci. Bulgarie 2, 437 (1957).Google Scholar
  9. 9.
    Koller, S.: Statistische Auswertung der Versuchsergebnisse. In Hoppe-Seyler/Thierfelders: Handbuch der physiologisch-chemischen Analyse, 10. Aufl., Bd. 2, S. 931. Berlin: Springer 1955.Google Scholar
  10. 10.
    Lamirande, G. de, C. Allard and A. Cantero: Ribonucleic Acid Composition of Rat Liver tumor Induced by 4-Dimethylaminoazobenzene. Cancer Res. 15, 329 (1955).Google Scholar
  11. 11.
    Leslie, I.: The Nucleic Acids of Tissues and Cells. In E. Chargaff and J. Davidson, The Nucleic Acids, vol. 2 p. 1–93. New York: Academic Press 1955.Google Scholar
  12. 12.
    Lison, L.: Histochimie et cytochimie animales, p. 1–607. Paris: Gauthiers-Villars 1953.Google Scholar
  13. 13.
    Loeb, H. G., and E. Dickinson: Observations on Animal Nucleic Acid Analysis with the Ogur-Rosen Procedure. Arch. Biochem. 57, 515 (1955).Google Scholar
  14. 14.
    Miller, J. A., and E. C. Miller: The Carcinogenic Aminoazo Dyes. Advanc. Cancer Res. 1, 339 (1953).Google Scholar
  15. 15.
    Miller, E. C. and J. A. Miller: Biochemical Investigations on Hepatic Carcinogenesis. J. nat. Cancer Inst. 15, Suppl., 1571 (1955).Google Scholar
  16. 16.
    Ogur, M., and G. Rosen: The Nucleic Acids of Plant Tissues. I. The Extraction and Estimation of Desoxypentose Nucleic Acid and Pentose Nucleic Acid. Arch. Biochem. 25, 262 (1950).Google Scholar
  17. 17.
    Opie, E. L.: Mobilisation of Basophile Substance (Ribonucleic Acid) in the Cytoplasm of Liver Cells with the Production of Tumors by Butter Yellow. J. exp. Med. 84, 91 (1946).Google Scholar
  18. 18.
    Price, J. M., E. C. Miller and J. A. Miller: The Intracellular Distribution of Protein, Nucleic Acids, Riboflavin and Protein-bound Aminoazo Dye in the Livers of Rats Fed p-Dimethylaminoazobenzene. J. biol. Chem. 173, 345 (1948).Google Scholar
  19. 19.
    Price, J. M., E. C. Miller, J. A. Miller and G. M. Weber: Studies on the Intracellular Composition of Livers from Rats Fed Various Aminoazo Dyes. I. Cancer Res. 9, 398 (1949).Google Scholar
  20. 20.
    Price, J. M., E. C. Miller, J. A. Miller and G. M. Weber: Studies on the Intracellular Composition of Livers from Rats Fed Various Aminoazo Dyes. II. Cancer Res. 10, 18 (1950).Google Scholar
  21. 21.
    Price, J., J. Harmann, E. Miller, and J. Miller: Progressive Microscopic Alterations in the Livers of Rats Fed the Hepatic Carcinogens 3′-Methyl-4-Dimethylaminoazobenzene and 4′-Fluoro-4-Dimethylaminoazobenzene. Cancer Res. 12, 192 (1952).Google Scholar
  22. 22.
    Thomson, R. Y., F. C. Heagy, W. C. Hutchinson and J. N. Davidson: The Deoxyribonucleic Acid Content of the Rat Cell Nucleus and its Use in Expressing the Results of Tissue Analysis, with Particular Referance to the Composition of Liver Tissue. Biochem. J. 53, 460 (1953).Google Scholar

Copyright information

© Springer-Verlag 1958

Authors and Affiliations

  • A. A. Hadjiolov
    • 1
  • Z. S. Tentschewa
    • 1
  1. 1.Biochemischen Institut der Medizinischen Fakultät in SofiaBulgarien

Personalised recommendations