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Chitosome-like vesicles from gamma particles of Blastocladiella emersonii synthesize chitin

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Abstract

When gamma particles isolated from the aquatic fungus, Blastocladiella emersonii, were incubated in a supernatant derived from a homogenate of zoospores previously triggered to encyst with 50 mM KCl, they exhibited a three-fold increase in chitin synthetase activity and produced chitosome-like vesicles. Passage of such vesicles through a linear sucrose gradient resulted in a symmetrical distribution of chitin synthetase activity over a broad portion of the gradient, and the specific activity of the peak fraction was seven times greater than that of the gamma particles. After isopycnic sucrose gradient centrifugation, chitin synthetase activity occurred in a band of particles with a peak buoyant density of 1.18 g/cm3. Ultrastructural examination of negatively stained samples from the peak fraction revealed spheroidal, chitosome-like particles 70–120 nm in diameter. Suspension of these particles produced chitin microfibrils when incubated with uridine diphosphate N-acetylglucosamine, the substrate for chitin synthetase.

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Abbreviations

GlcNAc:

N-acetylglucosamine

UDP-GlcNAc:

uridine diphosphate N-acetylglucosamine

PYG agar:

1.25 g of peptone, 1.25 g of yeast extract, 3 g of glucose, and 20 of agar per 1000 ml of water, the pH being adjusted to 6.8 with KOH after autoclaving

EGTA:

ethyleneglycol-bis (β-aminoethylether)-N,N′-tetraacetic acid

References

  • Barstow WE, Pommerville J (1980) The cytology of cell wall formation during zoospore development in Allomyces macrogynus. Arch Microbiol 128:179–189

    Google Scholar 

  • Bartnicki-Garcia S, Bracker CE, Reyes E, Ruiz-Herrera J (1978) Isolation of chitosomes from taxonomically diverse fungi and synthesis of chitin microfibrils in vitro. Exp Mycol 2:173–192

    Google Scholar 

  • Bracker CE, Ruiz-Herrera J, Bartnicki-Garcia S (1976) Structure and transformation of chitin synthetase particles (chitosomes) during microfibril synthesis in vitro. Proc Nat Acad Sci USA 73:4570–4574

    Google Scholar 

  • Cantino EC, Hyatt MT (1953) Phenotypic “sex” determination in the life history of a new species of Blastocladiella, B. emersonii. Antonie Van Leeuwenhoeck J Microbiol Serol 19:25–70

    Google Scholar 

  • Cantino EC, Mack JP (1969) Form and function in the zoospore of Blastocladiella emersonii I. The gamma particle and satelite ribosome package. Nova Hedwigia 18:115–148

    Google Scholar 

  • Giddings TH, Cantino EC (1974) Partial purification and properties of d-glucosamine 6-phosphate N-acetyltransferase from zoospores of Blastocladiella emersonii. J Bacteriol 120:976–979

    Google Scholar 

  • Gomes SL, Mennucci L, Costa Maia JC (1980) Calcium efflux during germination of Blastocladiella emersonii. Dev Biol 77:157–166

    Google Scholar 

  • Hutchinson TE, Cantino ME, Cantino EC (1977) Calcium is a prominent constituent of the gamma particle in the zoospore of Blastocladiella emersonii as revealed by x-ray microanalysis. Biochem Biophys Res Commun 74:336–342

    Google Scholar 

  • Jen CJ, Haug A (1979) Changes in surface properties of developing zoospores of Blastocladiella emersonii: binding of Concanavalin A. J Gen Microbiol 115:145–151

    Google Scholar 

  • Leonards KS, Haug A (1980) Effects of cations on the plasma membrane of Blastocladiella emersonii zoospores. Biochim Biophys Acta 600:805–816

    Google Scholar 

  • Lovett JS (1975) Growth and differentiation of the water mold Blastocladiella emersonii: Cytodifferentiation and the role of ribonucleic acid and protein synthesis. Bacteriol Rev 39:345–404

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Myers RB, Cantino EC (1974) The gamma particle. A study of cellorganelle interactions in the development of the water mold Blastocladiella emersonii. In: Wolsky A (ed) Monographs in Developmental Biology, vol 8. S. Karger, Basel

    Google Scholar 

  • Mills GL, Cantino EC (1978) The lipid composition of the Blastocladiella emersonii gamma particle and the function of gamma-particle lipid in chitin formation. Exp. Mycol 2:99–109

    Google Scholar 

  • Norrman J, Myers RB, Giddings TH, Cantino EC (1973) Partial purification of l-glutamine: d-fructose 6-phosphate aminotransferase from zoospores of Blastocladiella emersonii. Biochim Biophys Acta 302:173–177

    Google Scholar 

  • Norrman J, Giddings TH, Cantino EC (1975) Partial purification and properties of l-glutamine: d-fructose 6-phosphate aminotransferase from zoospores of Blastocladiella emersonii. Phytochemistry 14:1271–1274

    Google Scholar 

  • Ruiz-Herrera J, Sing VO, Van der Woude WJ, Bartnicki-Garcia S (1975) Microfibril assembly by granules of chitin synthetase. Proc Nat Acad Sci USA 72:2706–2710

    Google Scholar 

  • Ruiz-Herrera J, Lopez-Romero E, Bartnicki-Garcia S (1977) Properties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii. J Biol Chem 252:3338–3343

    Google Scholar 

  • Selitrennikoff CP, Allin D, Sonneborn DR (1976) Chitin biosynthesis during Blastocladiella zoospore germination: Evidence that the hexosamine biosynthetic pathway is post-translationally activated during cell differentiation. Proc Nat Acad Sci USA 73:534–538

    Google Scholar 

  • Selitrennikoff CP, Sonneborn DR (1976a) Post-translational control of de novo cell wall formation during Blastocladiella emersonii zoospore germination. Feedback regulation of hexosamine biosynthesis. Dev Biol 54:37–51

    Google Scholar 

  • Selitrennikoff CP, Sonneborn DR (1976b) The last two pathway-specific enzyme activities of hexosamine biosynthesis are present in Blastocladiella emersonii zoospores prior to germination. Biochim Biophys Acta 451:408–416

    Google Scholar 

  • Soll DR, Sonneborn DR (1969) Zoospore germination in the water mold, Blastocladiella emersonii II. Influence of cellular and environmental variables on germination. Dev. Biol 20:218–235

    Google Scholar 

  • Soll DR, Sonneborn DR (1972) Zoospore germination in Blastocladiella emersonii IV. Ion control over cell differentation. J Cell Sci 10: 315–333

    Google Scholar 

  • Suberkropp KF, Cantino EC (1972) Environmental control of motility and encystment in Blastocladiella emersonii zoospores at high population densities. Trans Br Mycol Soc 59:463–475

    Google Scholar 

  • Truesdell LC, Cantino EC (1970) Decay of gamma particles in germinating zoospores of Blastocladiella emersonii. Arch Mikrobiol 70:378–392

    Google Scholar 

  • Truesdell LC, Cantino EC (1971) The induction and early events of germination in the zoospore of Blastocladiella emersonii. In: Monroy A, Moscona AA (eds) Current Topics in Developmental Biology, vol 6. Academic Press Inc. New York, pp 1–44

    Google Scholar 

  • Van Brunt J, HaroldFM (1980) Ionic control of germination of Blastocladiella emersonii zoospores. J Bacteriol 141:735–744

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany and Plant Pathology, Michigan State University, 48824, East Lansing, Michigan, USA

    Gary L. Mills & Edward C. Cantino

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  1. Gary L. Mills
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  2. Edward C. Cantino
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Mills, G.L., Cantino, E.C. Chitosome-like vesicles from gamma particles of Blastocladiella emersonii synthesize chitin. Arch. Microbiol. 130, 72–77 (1981). https://doi.org/10.1007/BF00527075

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  • DOI: https://doi.org/10.1007/BF00527075

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