Zeitschrift für Krebsforschung

, Volume 68, Issue 1, pp 1–13 | Cite as

Studio quantitativo della crescita dell'epatoma-ascite di Yoshida AH 130

  • Giuseppe Bombara
  • Domenica Morabito
Article

Zusammenfassung

Bei gleichen Zeitintervallen nach Überimpfung von 20×106 Tumorzellen wurden die folgenden Parameter bestimmt: gesamte Tumorzellzahl, Ascites-Volumen, Mitose-Index, Tumorzell-Lebensfähigkeit (Schrek's Test) und prozentuale Zusammensetzung des Zellbildes.

Die Wachstumskurve ist durch drei Phasen charakterisiert: eine initiale, 3 Tage lange Phase rapider Zellteilung mit exponentiellem Verlauf (Anfangsphase), eine Verzögerungsphase mit absinkender Wachstumsrate, die bis zum 8.–9. Tag dauert (Zwischenphase), eine Phase gehemmter Zellproliferation, die mit dem Tode der Tiere endigt (Terminalphase). Der Mitose-Index ist höher in der Anfangsphase, vermindert in den folgenden Tagen bis zu kleinsten Werten in der Terminalphase. Die Zunahme des Ascites ist nicht proportional zu der Tumorzellvermehrung, aber sie ist intensiver nach den ersten Tagen und bis zur Terminalphase des Tumorwachstums. Die Prozentzahl der nicht vitalen Tumorzellen, die sich bei dem Schrek's Test mit Eosin anfärben, ist sehr niedrig und für die ganze Dauer des Tumorwachstums unverändert. Gleich nach dem Anfang der Tumorzellproliferation sind die Mastzellen vermindert und am 3.–4. Tag ganz verschwunden; während der ersten Tage sind neutrophile und eosinophile Granulocyten und wenige Lymphocyten und Histiocyten vermehrt. Der Kernpolymorphismus der Tumorzellen ist in der Anfangsphase am größten; «Pairs» (Yoshida) sind in der Terminalphase am häufigsten. Kein Unterschied in Tumorwachstum findet sich hinsichtlich des Geschlechtes. In jungen Ratten (90–100 g) dagegen ist das Wachstum, nach Zellzahl und Ascites-Volumen beurteilt, geringer als bei erwachsenen Tieren (180–240 g).

Summary

After equal intervals of time following the inoculation of 20×106 tumor cells these parameters were studied: the total number of tumor cells, the volume of ascites, the index of mitoses, the viability of tumor cells (Schrek's test), and the percentage distribution of the various cell types.

The growth-curve found is characterized by three phases: an initial, rapid division of cells lasting three days with an exponential course (beginning phase); a delayed phase with a declining rate of growth, which lasts up to the 8–9th day (intermediate phase); and a phase of inhibited cellular proliferation that ends with the death of the animal (terminal phase). The index of mitoses is higher in the beginning phase, and reduced in the subsequent days with the smallest values in the terminal phase. The increase of the ascites is not proportional to the increase in tumor cells, but is more intense after the first days up to the terminal phase of the tumor growth. The percentage of non-viable tumor cells, which stain in the Schrek's test with eosin, is very low and remains unchanged for the duration of the tumor growth. Immediately after the tumor cells begin to proliferate the mast cells become reduced in number, and on the third to fourth day disappear entirely. During the first days the neutrophils and eosinophils are increased, the lymphocytes and histiocytes only slightly. The nuclear polymorphism of the tumor cells is greatest at the beginning phase. “Pairs” (Yoshida) are most frequent in the terminal phase. No difference in the growth of the tumor is found between the sexes. In contrast, in young rats (90–100 g) the growth, as judged by the cell number and the volume of ascites, is less than in adult animals (180–240 g).

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Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • Giuseppe Bombara
    • 1
  • Domenica Morabito
    • 1
  1. 1.Istituto di Patologia Generale dell'Università di MessinaMessinaI'italia

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