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Über die Entwicklung des cytopathogenen Effektes durch drei verschiedene Polyoma-Virus-Linien in Mäuseembryonalkulturen

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Zusammenfassung

Zwischen der Wirkung von Polyoma-Virus BB-T-2 von Graffi einerseits und dem Polyoma-Virus von Stewart bzw. Rowe bestehen sichtbare Unterschiede des cytopathogenen Effektes (CPE), obwohl der infektiöse Titer für Fibroblastenkulturen bei allen drei Linien gleich hoch war. Das Graffi-Polyoma-Virus verursacht zumeist die sog. Defektform des CPE, welche relativ rasch eintritt und schnell zum Untergang der gesamten Zellkultur führt; das Stewart-Polyoma-Virus und das Rowe-Polyoma-Virus verursacht eine langsamer ablaufende sog. Rarefizierungsform des CPE, die schwierig von unspezifischen Veränderungen abzugrenzen ist.

Summary

There were visible differences in the cytopathogenic effect (CPE) between the Polyoma virus BB-T-2 of Graffi and that of Stewart or Rowe, although the infectious titer in fibroblastic cultures for all three strains was equally high. The Graffi Polyoma virus caused mostly the socalled defective form of the CPE, which occurred relatively quickly leading to a rapid destruction of the entire cell culture. The Stewart Polyoma virus and the Rowe Polyoma virus induced a more slowly developing, socalled rarefying form of the CPE which was difficult to differentiate from the non-specific changes.

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Literatur

  • Dawe, Cl. J., and L. W. Law: Morphologic changes in salivary-gland tissue of the newborn mouse exposed to parotid-tumor agent in vitro. J. nat. Cancer Inst. 23, 1157 (1959).

    Google Scholar 

  • —— and Th. B. Dunn: Studies of parotid-tumor agent in cultures of leukemia tissues of mice. J. nat. Cancer Inst. 23, 717 (1959).

    Google Scholar 

  • Eddy, B. E., S. E. Stewart and W. Berkeley: Cytopathogenicity in tissue cultures by tumor virus from mice. Proc. Soc. exp. Biol. (N. Y.) 98, 848 (1958).

    Google Scholar 

  • Graffi, A.: Persönliche Mitteilungen.

  • — J. Gimmy u. L. Krause: Über ein polyvalentes Sarkomvirus der Ratte. Naturwissenschaften 46, 330 (1959).

    Google Scholar 

  • Sanford, K. R., T. B. Dunn, A. B. Covalesky, L. T. Dupree and W. R. Earle: Polyoma viruses and production of malignancy in vitro. J. nat. Cancer Inst. 26, 331 (1961).

    Google Scholar 

  • Saxen, L., T. Vainio and S. Toivonen: Effect of polyoma virus on mouse kidney rudiment in vitro. J. nat. Cancer Inst. 29, 597 (1962).

    Google Scholar 

  • Stewart, S. E., B. E. Eddy and N. Borgese: Neoplasms in mice inoculated with a tumor agent carried in tissue culture. J. nat. Cancer Inst. 20, 1223 (1958).

    Google Scholar 

  • Vogt, M., and R. Dulbecco: Studies on cells rendered neoplastic by Polyoma virus: the problem of the presence of virus-related materials. Virology 16, 41 (1962).

    Google Scholar 

  • Wallace, R., M. C. Davies, B. Pease, M. Englert and A. W. Moyer: Propagation of mouse Polyoma virus in a strain of mouse mammary tumor cells in vitro. Proc. Soc. exp. Biol. (N. Y.) 103, 845 (1960).

    Google Scholar 

  • Youngner, J. S.: Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells. Proc. Soc. exp. Biol. (N. Y.) 85, 202 (1954).

    Google Scholar 

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Authors and Affiliations

  1. Pathologischen Institut der Universität München, München, Deutschland

    A. Georgii & H. Wrba

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  1. A. Georgii
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  2. H. Wrba
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Georgii, A., Wrba, H. Über die Entwicklung des cytopathogenen Effektes durch drei verschiedene Polyoma-Virus-Linien in Mäuseembryonalkulturen. Z Krebs-forsch 65, 582–589 (1963). https://doi.org/10.1007/BF00525234

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  • DOI: https://doi.org/10.1007/BF00525234

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