Summary
Estrone metabolizing enzymes of microsomes from normal rat liver, minimal deviation hepatomas (5123 C, 7800 and 9121) and a fast growing transplanted hepatoma (DENA-1) were assayed. The consumption of estrone-[16-14C], the formation of estradiol-(17β) and the formation of protein-bound and watersoluble metabolites was followed.
The fast growing hepatomas had lost the ability to inactivate estrone. The inactivation of estrone by the minimal deviation hepatomas tested was considerably slower as compared with normal liver.
Zusammenfassung
Der Stoffwechsel von Oestron-[16-14C] durch Mikrosomen von normaler Rattenleber, Minimalabweichungshepatomen (5123 C, 7800 und 5121) sowie einem schnell wachsenden Hepatom (DENA-1) wurde untersucht. Gemessen wurden der Substratverbrauch, die Bildung von Oestradiol-(17β) sowie von proteingebundenen und wasserlöslichen Metaboliten.
Während das schnell wachsende Hepatom die Fähigkeit zur Oestroninaktivierung vollständig verloren hat, zeigen die Minimalabweichungshepatome einen gegenüber Normalleber verminderten Oestrogenstoffwechsel.
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References
Adamson, R. H., and J. R. Fouts: The metabolism of drugs by hepatic tumors. Cancer Res. 21, 667 (1961).
Breuer, H., J. Breuer u. D. Schmähl: Stoffwechsel von Östradiol-17β in dem durch 7,12-Dimethyl-benzanthrazen induzierten Mammacarcinom der Ratte. Z. Krebsforsch. 67, 247 (1965).
Bullough, W. S.: The control of mitotic activity in adult mammalian tissues. Biol. Rev. 37, 307 (1962).
Conney, A. H., and A. Klutch: Increased activity of androgen hydroxylase in liver microsomes of rats pretreated with phenobarbital and other drugs. J. biol. Chem. 238, 1611 (1963).
Fleck, A., and H. N. Munroe: The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim. biophys. Acta (Amst.) 55, 571 (1962).
Hecker, E., u. F. Marks: Zum Stoffwechsel und Wirkungsmechanismus der Oestrogene VII: Die o-Hydroxylierung von Oestron in Rattenleber und ihre Beziehungen zur Proteinbindung sowie zu Hydroxylierungen in aliphatischen Positionen. Biochem. Z. 343, 211 (1965). (and earlier communications).
Jacobsen, H. I.: The fate of steroid estrogens in target tissues. In: On cancer and hormones, p. 303–320. Chicago 1962.
Johnes, V. E., and V. Korenchevsky: J. Geront. 1, 336 (1946) (quoted by G. Pincus).
Kuntzman, R., M. Jacobson, K. Schneidman, and A. H. Conney: Similarities between oxidative drug-metabolizing enzymes and steroidhydroxylases in liver microsomes. J. Pharmacol. exp. Ther. 146, 280 (1964).
—, D. Lawrence, and A. H. Conney: Michaelis constants for the hydroxylation of steroid hormones and drugs by rat liver microsomes. Molec. Pharm. 1, 163 (1965).
Lacassagne, A.: Examples of the action of steroid hormones on the development of certain experimental tumors. In: Hormonal steroids (L. Martini, and A. Pecile, eds), vol. 2, p. 379–390. New York 1965.
Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265 (1951).
Marks, F., u. E. Hecker: Zum Stoffwechsel und Wirkungsmechanismus der Oestrogene VIII: Biogenese und Stoffwechsel von 2-Hydroxyoestron in Beziehung zur Bildung proteingebundener und wasserlöslicher Oestronmetaboliten und zur NADPH-Oxydation in Rattenlebermikrosomen. Hoppe-Seylers Z. physiol. Chem. 345, 22 (1966).
Morris, H. P.: Studies on the development, biochemistry and biology of experimental hepatomas. Advanc. Cancer Res. 9, 227 (1965).
Murray, R. K., R. Süss, and H. C. Pitot: Studies on smooth and rough endoplasmic reticulum of liver and hepatoma. Fed. Proc. 25, Abstr. 973 (1966).
Nebert, D. W., and H. S. Mason: An electron spin resonance study of neoplasms. Cancer Res. 23, 833 (1963).
——: A microsomal difference between normal liver and “minimal-deviation” hepatoma 5123 detectable by electron spin resonance. Biochim. biophys. Acta (Amst.) 86, 415 (1964).
Pincus, G.: The physiology of ovarian hormones. In: The hormones (G. Pincus and K. V. Thimann eds.) vol. II, p. 1–31. New York 1950.
Reuber, M. D.: Importance of thyroid hormones and testosterone in the induction of carcinoma and cirrhosis of the liver in female Wistar rats ingesting N-2-fluorenyl-diacetamide. J. nat. Cancer Inst. 36, 775 (1966).
Sugimura, T., K. Ikeda, K. Hirota, M. Hozumi, and H. P. Morris: Chemical, enzymatic, and cytochrome assays of microsomal fraction of hepatomas with different growth rates. Cancer Res. 26, 1711 (1966).
Wolley, G. W., J. J. Harris, P. C. Merker, and M. N. Teller: Action of hormonal steroids on transplantable human and animal tumors. In: Hormonal steroids (L. Martin, and A. Pecile eds.), vol. 2, p. 361–366. New York 1965.
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We wish to thank Mrs. E. Kubinyi and Miss E. Staedtler for excellent technical assistance.
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Marks, F., Süss, R., Morris, H.P. et al. Metabolism and mechanism of action of estrogens. Z Krebs-forsch 69, 361–369 (1967). https://doi.org/10.1007/BF00525151
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DOI: https://doi.org/10.1007/BF00525151