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Comparative studies on the phosphatides of normal rat liver and primary hepatoma

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Summary

A low phosphatide content was found in primary hepatoma of rat when compared with normal rat liver. Chromatographic analyses did not reveal, however, remarkable differences in the phosphatide composition of the normal and neoplastic tissue. The ratio of the 32P incorporation in vitro into phosphatidylcholine and phosphatidylethanolamine was lower in normal rat liver than in primary liver tumour. Gas chromatographic analyses of fatty acids performed on total lipid and its different components disclosed also notable differences between hepatoma and normal liver. A significant difference was observed in the stearic acid-oleic acid proportions between phosphatides of rat liver and primary hepatoma. The plasmalogen content of hepatoma was relatively rather high. The comparative analysis of phosphatides was extended to cell fractions obtained by differential centrifugation and to a mitochondrial membrane fragment. Within one tissue the fatty acid composition appeared to be characteristic for the lipid component and was independent of the cell fraction. A similar 32P pattern of the phosphatides from all cell fractions of one tissue was found after an incubation of slices with radioactive phosphate. Consequently the observed differences between phosphatides of normal rat liver and hepatoma are distributed throughout the cells.

Zusammenfassung

Im primären Hepatom der Ratte (Verfütterung von Dimethylaminoazobenzol) wurde im Vergleich zu normaler Rattenleber ein erniedrigter Gehalt an Phosphatiden bei weitgehend gleichartiger Zusammensetzung gefunden. Während in normaler Leber die in vitro-Einbaurate von 32P-Phosphat in Phosphatidylcholin und in Phosphatidyläthanolamin etwa gleichartig hoch war, zeigte das Hepatom einen bevorzugten Einbau in Phosphatidylcholin. In der Zusammensetzung der Fettsäuren der Gesamt-Lipide und ihrer verschiedenen Komponenten (gaschromatographische Analysen) bestanden deutliche Unterschiede zwischen Leber und Hepatom, wobei besonders bemerkenswert im Hepatom die signifikante Zunahme der Ölsäure auf Kosten der Stearinsäure in der Phosphatidfraktion war. Der Plasmalogen-Gehalt im Hepatom war relativ erhöht. —Die vergleichende Analyse der Phosphatide wurde auch auf durch differentielle Zentrifugation erhaltene Zellfraktionen und auf Mitochondrienmembran-Fragmente ausgedehnt. Innerhalb eines Gewebes (Leber bzw. Hepatom) schien die Zusammensetzung der Fettsäuren charakteristisch für die Lipid-Komponente aber unabhängig, von der Zellfraktion zu sein. Nach Inkubation von Gewebsschnitten mit 32P-Phosphat wurde in allen Zellfraktionen eine ähnliche und dem Gesamtgewebe entsprechende 32P-Verteilung bei den Phosphatiden gefunden. Die beobachteten Unterschiede zwischen den Phosphatiden der normalen Rattenleber und des Hepatoms sind demnach bei beiden Geweben in der ganzen Zelle gleichmäßig vorhanden.

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Authors and Affiliations

  1. Laboratory of Organic Chemistry, State University of Utrecht, The Netherlands

    J. H. Veerkamp, I. Mulder & L. L. M. van Deenen

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  1. J. H. Veerkamp
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  2. I. Mulder
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  3. L. L. M. van Deenen
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Additional information

Contribution No 14 in the series: Metabolism and functions of phosphatides. This work is part of the thesis of J. H. Veerkamp, Utrecht 1960.

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Veerkamp, J.H., Mulder, I. & van Deenen, L.L.M. Comparative studies on the phosphatides of normal rat liver and primary hepatoma. Z Krebs-forsch 64, 137–148 (1961). https://doi.org/10.1007/BF00525080

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  • DOI: https://doi.org/10.1007/BF00525080

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