Zeitschrift für Krebsforschung

, Volume 61, Issue 5, pp 578–586 | Cite as

Einwirkung von Stickstofflost, seinem N-Oxyd, Colchicin und Röntgenstrahlen auf Nucleinsäuren und deren Bausteine

  • Christoph Scholtissek


  1. 1.

    The migration rate of nucleic acids in paper electrophoresis toward the positive pole is decreased by nitrogen mustard (I) and its N-oxide derivative (II). The fractions, however, are not distinct, due to tail formation. Colchicine and X-rays have no effect.

  2. 2.

    The ability to stain nucleic acids with methylgreen-pyronin disappears after the reaction with I or II. This loss of staining properties is evident even before the alteration of the electrophoretic behavior is observed. Treatment with X-rays causes more intensive staining. Colchicine has no effect. The failure of staining may be explained by a blocking of the acid groups. On the other hand, X-rays seem to increase the number of acid groups by the splitting of the macromolecule.

  3. 3.

    Reactions of I and II using some model substances (ATP, AMP, glucose-6-phosphate, adenine, adenosine, inorganic phosphate) yield numerous products in each case (probably through substitution in the purine nucleus). Inorganic phosphate is not split off by the reaction of I of II with ATP, AMP and glucose-6-phosphate.

  4. 4.

    The method mentioned above, in (1) and (2), my be applied in the study of other substances with alkylating or degradating effects on nucleic acids under physiological conditions (radiomimetic substances).



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  1. 1.
    Alexander, P.: Interference with the formation of a nucleoprotein complex by radiomimetic compounds. Nature (Lond.) 169, 226 (1952).Google Scholar
  2. 1a.
    —: Radrochemische Reaktionen und Radiobiologie. Vortrag Max-Planck-Institut für medizinische Forschung, Heidelberg, 22. Okt. 1956.Google Scholar
  3. 2.
    Burrows, S., F. S. M. Grylls and J. S. Harrison: Paper chromatography of phosphoric esters. Nature (Lond.) 170, 800 (1952).Google Scholar
  4. 3.
    Butler, J. A. V., L. A. Gilbert and K. A. Smith: Radiomimetic action of sulphur and nitrogen “mustards” on deoxyribonucleic acid. Nature (Lond.) 165, 714 (1950).Google Scholar
  5. 4.
    Butler, J. A. V., and K. A. Smith: The aciton of ionizing radiations and of radiomimetic substances on deoxyribonucleic acid. Part I. The action of some compounds of the “mustard” type. J. Chem. Soc. (Lond.) 1950, 3411.Google Scholar
  6. 5.
    Butler, J. A. V., L. Gilbert, D. W. James and W. C. J. Ross: Degradation of deoxyribonucleic acid by a “nitrogen mustard”. Nature (Lond.) 168, 985 (1951).Google Scholar
  7. 6.
    Butler, J. A. V., L. Gilbert and D. W. F. James: The action of ionizing radiations and of radiomimetic substances on deoxyribonucleic acid. Part VI. Physicochemical mesurements of the action of bischloroethylmethylamin. J. Chem. Soc. (Lond.) 1952, 3268.Google Scholar
  8. 7.
    Chanutin, A., and E. C. Gjessing: The effect of nitrogen mustards upon the ultraviolet absorption spectrum of thymonucleate, uracil and purins. Cancer Res. 6, 599 (1946).Google Scholar
  9. 8.
    Conway, B. E., L. Gilbert and J. A. V. Butler: The action of ionizing radiations and of radiomimetic substances on deoxyribonucleic acid. Part III. The molekular weights of deoxyribonucleic acid degraded by x-rays and by treatment with a “nitrogen mustard”. J. Chem. Soc. (Lond.) 1950, 3421.Google Scholar
  10. 9.
    Deimel, M., M. Maurer u. W. Maurer: Papierelektrophoretische Trennung von Ribo-und Desoxyribonucleinsäure. Naturwiss. 39, 489 (1952).Google Scholar
  11. 10.
    Elmore, D. T., J. M. Gulland, D. O. Jordan and H. F. W. Taylor: The reaction of nucleic acids with mustard gas. Biochemic. J. 42, 308 (1948).Google Scholar
  12. 11.
    Fruton, J. S., W. H. Stein, M. A. Stahmann and C. Golumbic: Chemical reactions of the nitrogen mustard gases. VI. The reactions of the nitrogen mustard gases with chemical compounds of biological interest. J. of Org. Chem. 11, 571 (1946).Google Scholar
  13. 12.
    Golumbic, C., J. S. Fruton and M. Bergmann: Chemical reactions of the nitrogen mustard gases. I. The transformations of methyl-bis (β-chloroethyl)amine in water. J. of Org. Chem. 11, 518 (1946).Google Scholar
  14. 13.
    Gurin, S., A. M. Delluva and D. I. Crandall: Chemical reactions of nitrogen mustard gases. II. Reactions with secondary and tertiary amines. J. of Org. Chem. 12, 612 (1947).Google Scholar
  15. 14.
    Gurin, S., A. M. Delluva and D. I. Crandall: Chemical reactions of nitrogen mustard gases. I. Reactions of methyl-bis (β-chloroethyl)amine with Hexamethylene tetramine. J. of Org. Chem. 12, 606 (1947).Google Scholar
  16. 15.
    Hanes, C. S., and F. A. Isherwood: Separation of the phosphoric esters on the filter paper chromatogramm. Nature (Lond.) 164, 1107 (1949).Google Scholar
  17. 16.
    Hirst, R., u. R. Bechtold: Biophysikalische Studien mit dem Tensiometer. Arzneimittel-Forsch. 3, 175 (1953).Google Scholar
  18. 17.
    Kanngiesser, W.: Über die Papierelektrophorese von Nucleinsäuren. Naturwiss. 38, 503 (1951).Google Scholar
  19. 18.
    Lettré, H.: Zum Mechanismus der cytostatischen Wirkung des N-Losts. Naturwiss. 41, 188 (1954).Google Scholar
  20. 19.
    Lettré, H., u. R. Letté: Aufhebung der Wirkung von Mitosegiften durch chemische Faktoren. Naturwiss. 33, 283 (1946).Google Scholar
  21. 20.
    Markham, R., and J. D. Smith: Chromatographic studies of nucleic acids. Biochemic. J. 49, 401 (1951).Google Scholar
  22. 21.
    Pelzer, H.: Diplomarbeit Univ. Mainz 1953. Zit. nach F. Turba u. H. Schuster, Enzymatische Synthese von C14-markierter Adenosintriphosphorsäure. Biochem. Z. 325, 537 (1954).Google Scholar
  23. 22.
    Scholtissek, C.: Einwirkung von Stickstofflost und Colchicin auf Serumeiweiße. Z. Krebsforsch. 61, 309 (1956).Google Scholar
  24. 23.
    Schümmelfeder, N., u. W. Heyer: Papierelektrophoretische Untersuchungen an Nucleinsäuren. Naturwiss. 41, 164 (1954).Google Scholar
  25. 24.
    Vischer, E., and E. Chargaff: The separation and characterization of purins in minute amounts of nucleic acid hydrolyzates. J. of Biol. Chem. 168, 781 (1947).Google Scholar

Copyright information

© Springer-Verlag 1957

Authors and Affiliations

  • Christoph Scholtissek
    • 1
  1. 1.Institut für experimentelle Krebsforschung der Universität HeidelbergHeidelbergDeutschland

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