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Über die Bedeutung der freien SH-Gruppen für die Aktivität des Agens des Rous-Sarkoms und des Shope-Papillom-Virus

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Summary

It is reported on experiments with the Rous-sarcoma and the Shopepapilloma. The agent of the Rous-sarcoma can bei inactivated by the SH-blocker p-Chloromercuribenzoic acid. This inactivation proved to be largely reversible by a overplus of Cysteine as SH-donator.

Thereby was showed, that the agent of Rous-sarcoma contains free SH-groups. By the reactivation with Cysteine was proved, that in this loss of activity does not lie before a toxic denaturation by the p-Chloromercuribenzoic acid, but the place of attack are the free Sh-groups.

The completeness of the loss of activity shows, that these free SH-groups are necessary for formation of tumor.

These resultates tend to show further, that the free SH-groups play a important role in the intracellular binding of the agent. The inactivation of the Rous-agent by oxidation may attack at least in part, if not even exclusively in the free SH-groups.

For the virus of Shope-papilloma could not be proved by the employed method free, for activity needed SH-groups.

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  1. Arbeitsbereich Biochemie, Geschwulstlabor, Institut für Medizin und Biologie der Deutschen Akademie der Wissenschaften, Berlin-Buch, Berlin-Buch, Deutschland

    Ferdinand Schmidt

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Schmidt, F. Über die Bedeutung der freien SH-Gruppen für die Aktivität des Agens des Rous-Sarkoms und des Shope-Papillom-Virus. Z Krebs-forsch 61, 527–533 (1957). https://doi.org/10.1007/BF00524629

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  • DOI: https://doi.org/10.1007/BF00524629

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