Zeitschrift für Krebsforschung

, Volume 62, Issue 2, pp 116–124 | Cite as

The metabolism of neoplastic tissues: The metabolic activity of normal and cancerous rat livers studied with acetate-1-14C in vitro

  • P. Emmelot
  • G. H. van Vals


In vitro studies with surviving tissue slices show that neither the activation nor the utilization of acetate-1-14C in four important metabolic sequences is impaired in the neoplastic rat liver following the feeding of p-dimethylaminoazobenzene. Incorporation of 14C from acetate-1-14C into the proteins is very markedly enhanced in the hepatoma and hepatoma-bearing liver as compared with that in the normal and precancerous liver. It is concluded that the citric acid cycle is markedly active in the neoplastic rat liver.


Public Health Cancer Research Citric Acid Metabolic Activity Vitro Study 
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  1. 1.
    Zamecnik, P. C., R. B. Loftfield, M. C. Stephenson and J. M. Steele: Studies on the carbonhydrate and protein metabolism of the rat hepatoma. Cancer Res. 11, 592–602 (1951).Google Scholar
  2. 2.
    Campbell, P. N.: Utilization of glucose for the synthesis of protein in chicken and rat liver and rat hepatoma in vitro. Biochemic. J. 61, 496–503 (1955).Google Scholar
  3. 3.
    Olson, R. E.: Oxidation of C14-labeled carbohydrate intermediates in tumor and normal tissue. Cancer Res. 11, 571–584 (1951).Google Scholar
  4. 4.
    Medes, G., B. Friedmann and S. Weinhouse: Acetate metabolism in vitro during hepatocarcinogenesis by p-dimethylaminoazobenzene. Cancer Res. 16, 57–62 (1956).Google Scholar
  5. 5.
    Emmelot, P., and L. Bosch: The metabolism of neoplastic tissues: Synthesis of cholesterol and fatty acids from acetate by transplanted mouse tumours in vitro and in vivo. Brit. J. Canc. 9, 327–338 (1955).Google Scholar
  6. 6.
    Wyshak, G. H., and I. L. Chaikoff: Metabolic defects in the liver of fasted rats as shown by utilization of C14-labeled glucoses and fructose. H. of Biol. Chem. 200, 851–857 (1953).Google Scholar
  7. 7.
    Lyon, I., M. S. Masri and I. L. Chaikoff: Fasting and hepatic lipogenesis from C14-acetate. J. of Biol. Chem. 196, 25–32 (1952).Google Scholar
  8. 8.
    Masri, M. S., I. Lyon and I. L. Chaikoff: Nature of the stimulating action of insulin on lipogenesis from acetate in fasted rat liver. J. of Biol. Chem. 197, 621–624 (1952).Google Scholar
  9. 9.
    Emmelot, P., and L. Bosch: The metabolism of neoplastic tissues: The relation of carbohydrate utilization to cholesterol and fatty acid synthesis in tumour tissue slices. Brit. J. Canc. 9, 339–343 (1955).Google Scholar
  10. 10.
    Nakatani, H., K. Nakano and Y. Ohara: Untersuchung über den Gewebsstoffwechsel beim Verlauf der Leberkrebsentstehung durch Dimethylaminoazobenzolfütterung. Gann (jap.) 32, 240–244 (1938).Google Scholar
  11. 10a.
    Burk, D.: In: Symposium on Respiratory Enzymes, Madison: University Wisconsin Press 1942.Google Scholar
  12. 11.
    Burke, W. T., and L. L. Miller: Amino acid metabolism in perfused livers of rats with cirrhosis and azo dye-fed rats. Cancer Res. 16, 330–337 (1956).Google Scholar
  13. 12.
    Christensen, H. N., J. T. Rothwell, R. A. Sears and J. A. Streicher: Association between rapid growth and elevated cell concentrations of amino acids (in regenerating liver after partial hepatectomy in the rat). J. of Biol. Chem. 175, 101–105 (1948).Google Scholar
  14. 13a.
    Christensen, H. N., and J. A. Streicher: Association between rapid growth and elevated cell concentrations of amino acids (in fetal tissues). J. of Biol. Chem. 175, 95–100 (1948).Google Scholar
  15. 13.
    Dickens, F., and H. Weil-Marherbe: A comparison of the metabolism of tumors of liver and skin with that of the tissue of origin. Cancer Res. 3, 73–87 (1943).Google Scholar
  16. 14.
    Rutman, R. J., A. Cantarow and K. E. Paschkis: The in vitro uptake of alanine-1-C14 by preneoplastic liver and hepatoma mitochondrial protein. Cancer Res. 14, 115–118 (1954).Google Scholar
  17. 15.
    Zamecnik, P. C., I. D. Frantz, R. B. Loftfield and M. C. Stephenson: Incorporation in vitro of radioactive carbon from carboxyl-labeled dl-alanine and glycine into proteins of normal and maligmant rat livers. J. of Biol. Chem. 175, 299–314 (1948).Google Scholar
  18. 15a.
    Winnick, T.: Studies on the mechanism of protein synthesis in embryonic and tumor tissues. I. Evidence relating to the incorporation of labeled amino acids into protein structure in homogenates. Arch. of Biochem. 27, 65–74 (1950).Google Scholar
  19. 16.
    Price, J. M., J. A. Miller, E. C. Miller and G. M. Weber: The intracellular composition of liver and liver tumor from rats fed 4-dimethylaminoazobenzene. Cancer Res. 9, 96–102 (1949).Google Scholar
  20. 16a.
    Allard, C., G. de Lamirande and A. Cantero: Mitochondrial population of mammalian cells: III Number of mitochondria per average cell of rat liver tumor induced by 4-dimethylaminoazobenzene. Significance in the comparative study of the mitochondrial fraction properties of normal tissues and tumors. Canad. J. Med. Sci. 31, 103–108 (1953).Google Scholar
  21. 17.
    Emmelot, P., and P. J. Brombacher: Enzymic activities of tumour mitochondria. Pyridine nucleosidases and amination processes. Biochim. et Biophysica Acta 22, 487–494 (1956).Google Scholar

Copyright information

© Springer-Verlag 1957

Authors and Affiliations

  • P. Emmelot
    • 1
  • G. H. van Vals
    • 1
  1. 1.Department of BiochemistryAnroni van Leeuwenhoek-Huis: The Netherlands Cancer InstituteAmsterdamthe Netherlands

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