Summary
The urines of two healthy persons and of two patients with bladder carcinoma were analysed in two different methods with regard to possible conjugates of tryptophan metabolites. In both cases the metabolites in question were to be found in free form. The tryptophan metabolites, eliminated in urine, are determinated in healthy individuals (20 to 30 years of age). The same examinations are carried out on patients with urinary bladder carcinoma. The elimination of one or more metabolites is found increased.
Bladder carcinoma are induced in rats through the implantation of cholesterol pellets which contained 4–5 mg 3-hydroxy-anthranilic acid. 30 rats were treated and after a period of 8–10 months it was found that 25 rats had developed tumors; 7 of which were carcinoma.
3-hydroxy-anthranilic acid-[3H] was instilled into the bladder of healthy rats. 20 to 30% were absorbed within 2 to 6 hours. The metabolite was not deposited into the bladder wall itself. After the intraveneous injection of 3-hydroxy-anthranilic acid-[3H] into rats, bearing bladder tumors, it was found to be incorporated in the tumor tissue, where its presence was clearly shown in radiohistological studies. A patient without bladder ailments who was given 3-hydroxy-anthranilic acid-[3H] eliminated 80% of the dosis within 3 days, while a patient with bladder carcinoma eliminated barely 50%.
Zusammenfassung
Der Urin von 2 normalen Personen und von 2 Trägern von Blasencarcinomen wurde auf Konjugatbildungen von Tryptophan-Metaboliten (Kynurenin, 3-Hydroxy-kynurenin, Kynurensäure, Xanthurensäure, Anthranilsäure und 3-Hydroxyanthranilsäure) nach zwei verschiedenen Methoden untersucht. In beiden Fällen lagen die untersuchten Metaboliten in freier Form vor. Bei Blasencarcinom-Patienten wurde eine erhöhte Ausscheidung eines oder mehrerer Metaboliten beobachtet.
Durch Implantation von Cholesterinkugeln, die 4–5 mg 3-Hydroxy-anthranilsäure enthalten, in die Harnblase von Ratten werden bei 30 Tieren nach 8–10 Monaten Inkubationsdauer 25 Tumoren, darunter 7 Carcinome, ausgelöst.
Nach Instillation von 3-Hydroxy-anthranilsäure-[3H] in die Blase gesunder Ratten werden innerhalb von 2–6 Std 20–30% resorbiert, die Blasenwand selbst zeigt keinen Einbau der Substanz. Nach i.v. Injektion von 3-Hydroxy-anthranilsäure-[3H] zeigt sich bei Ratten, die einen Blasentumor tragen, ein Einbau in den Tumor, der u. a. im radiohistologischen Schnitt eindeutig nachgewiesen werden kann. Bei einem blasengesunden Patienten wird 3-Hydroxy-anthranilsäure-[3H] i.v. injiziert. Nach 3 Tagen sind 80% der Dosis im Urin ausgeschieden worden. Bei einem Blasencarcinom-Patienten betrug die Ausscheidung nur knapp 50%.
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Kochen, W., Hochberg, K. Untersuchungen über den Tryptophan-Stoffwechsel beim Blasen-Carcinom. Z Krebs-forsch 73, 251–264 (1970). https://doi.org/10.1007/BF00524406
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DOI: https://doi.org/10.1007/BF00524406