Summary
A study was made of the behaviour of a number of non-ionic surface active tumour promoting agents (sorbitan fatty acid esters, and polyoxyethylene sorbitan fatty acid esters) towards cytochrome oxydase, employing the Warburg respirometer. Derivatives of the same compounds, as well as one anionic and one cationic detergent, and croton oil were investigated too.
Indications were given why the present study was carried out.
It was demonstrated that potent non-ionic surface active tumour promoters do not inhibit cytochrome oxydase in vitro. On the other hand, however, certain derivatives of the agents investigated, when used anone, were capable of more or less inactivating the enzyme.
The following points are discussed: the investigation technique, the significance of the observations on the behaviour of the compounds examined, and the possible nature of the intercation between synthetic detergents and proteins. It is concluded, that merely the formation of micellar aggregates (=solubilization), which is characteristic of surface active agents in general, does not per se cause inhibition of the enzyme and, further, that in cases in which inhibition occurs (ionic detergents) it is due to other properties of the surface active agents.
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Diese Untersuchung wurde teilweise unterstützt durch das Forschungsstipendium C-2930 M & G von National Institutes of Health, Public Health Service, nach Empfehlung des National Advisory Cancer Council (USA).
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Setälä, K., Lundbom, S. & Holsti, P. Über das Verhalten der nichtionisierbaren oberflächenaktiven tumorauslösenden Substanzen zu der Cytochromoxydase. Z Krebs-forsch 61, 681–687 (1957). https://doi.org/10.1007/BF00524396
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DOI: https://doi.org/10.1007/BF00524396