Ultrastructural studies on the islets of langerhans of the carp

  • Mitusuo Nakamura
  • Motoyoshi Yokote


The carp islet tissues are fixed with three kinds of fixation procedures (fixation with our modification of Dalton's fixative, with phosphate buffered osmium tetroxide and with glutaraldehyde followed by postosmication) and examined by electron microscopy and by correlative survey of consecutive thin and thick sections for electron and light microscopy. The results are as follows. 1. The islet tissues of the carp contain four types of glandular cells; A, B, clear and D cells. 2. It is strongly suggested that the clear cell is a variety of the B cell.


Islet of Langerhans Cell types Fish Cyprinus carpio 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bencosme, S. A., Meyer, J., Bergman, B. J., Martinetz-Palomo, A.: The principal islet of bull-head fish (Ictalurus nebulosus). A correlative light and electron microscopic study of islet cells and their secretory granules isolated by centrifugation. Rev. Canad. Biol. 24, 141–154 (1965).Google Scholar
  2. Bowie, D. J.: Studies of the islets of Langerhans in a teleost. Anat. Rec. 29, 57–71 (1924).Google Scholar
  3. Caramia, F.: Electron microscopic description of a third cell type in the islets of the pancreas of the rat. Amer. J. Anat. 112, 53–64 (1963).Google Scholar
  4. — Munger, B. L., Lacy, P. E.: The ultrastructural basis for the identification of cell types in the pancreatic islets. I Guinea pig. Z. Zellforsch. 67, 533–546 (1965).Google Scholar
  5. Falkmer, S.: Experimental diabetes research in fish. On the morphology and physiology of the endocrine pancreatic tissue of the marine teleost Cottus scorpius with special reference to the role of glutathion in the mechanism of alloxan diabetes using a modified nitroprusside method. Acta endocr. (Kbh.) 59 Suppl. (1961).Google Scholar
  6. — Hellman, B., Voigt, G. E.: On the agranular cells in the pancreatic islet tissue of the marine teleost Cottus scorpius. Acta path. microbiol. scand. 60, 47–54 (1964).Google Scholar
  7. — Olsson, R.: Ultrastructure of the pancreatic islet tissue of normal and alloxan-treated Cottus scorpius. Acta endocr. (Kbh.) 39, 32–46 (1962).Google Scholar
  8. Faller, A., Lange, R.: Kombinierte licht-und elektronenmikroskopische Analyse des Zellbildes in den Langerhansschen Inseln des Karpfens. Arch. histol. jap. 31, 73–80 (1969).Google Scholar
  9. Grosso, L. L.: The effect of alloxan on the pancreas, liver and kidney of the teleost, Lebistes reticulatus, with notes on the normal pancreas. Zoologica 35, 169–180 (1950).Google Scholar
  10. Kafuku, T.: Morphological differences between domesticated common carp and wild one. Speculation on the process of differentiation of the two carp races. Bull. Freshwater Fish. Res. Lab. 16, 71–82 (1966).Google Scholar
  11. Kobayashi, K.: Electron microscope studies on the Langerhans islets in the toad pancreas. Arch. histol. jap. 26, 439–482 (1966).Google Scholar
  12. — Takahashi, Y.: Light and electron microscope observations of the islets of Langerhans in Carassius carassius longsdorfii. Arch. histol. jap. 31, 433–454 (1970).Google Scholar
  13. Legg, P. G.: The fine structure and innervation of the beta and delta cells in the islet of Langerhans of the cat. Z. Zellforsch. 80, 307–321 (1967).Google Scholar
  14. Like, A. A.: The ultrastructure of the secretory cells of the islets of Langerhans in man. Lab. Invest. 16, 937–951 (1967).Google Scholar
  15. Like, A. A. Tashima, L. S., Cahill, G. F.: The giant islet of the toad fish, Opsamus tau L., an electron microscopic study. Internat. Congr. Series 74; Fifth Congr. of the Internat. Diabetes Federation 68 (1964).Google Scholar
  16. Munger, B. L., Caramia, F., Lacy, P. E.: The ultrastructural basis for the identification of cell types in the pancreatic islets. II. Rabbit, dog and opossum. Z. Zellforsch. 67, 776–798 (1965).Google Scholar
  17. Murrel, L. R., Nace, P. F.: Experimental diabetes in the catfish: normal and alloxan-diabetic blood glucose and pancreatic histology. Endocrinology 64, 542–550 (1959).Google Scholar
  18. Nace, P. F.: The endocrine pancreas of the toad fish, intact and treated with alloxan or cortisone. Anat. Rec. 124, 340 (1956).Google Scholar
  19. Nakamura, M., Yamada, K., Yokote, M.: Ultrastructural aspects of the pancreatic islets in carps of spontaneous diabetes mellitus. Experientia (in press).Google Scholar
  20. Reynolds, E.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).Google Scholar
  21. Sato, T., Herman, L., Fitzgerald, P. J.: The comparative ultrastructure of the pancreatic islet of Langerhans. Gen. comp. Endocr. 7, 132–157 (1966).Google Scholar
  22. Sorensen, R. L., Lindall, A. W., Lararow, A.: Studies on the isolated goosefish insulin secretion granule. Diabetes 18, 129–137 (1969).Google Scholar
  23. Stempak, J. H., Ward, R. T.: An improved staining method for electron microscopy. J. Cell Biol. 22, 697–701 (1964).Google Scholar
  24. Titlbach, M.: Feinstruktur der Zellen der Langerhansschen Inseln bei Cyprinus carpio L. Z. mikr.-anat. Forsch. 75, 184–197 (1966).Google Scholar
  25. —: Licht- und elektronenmikroskopische Untersuchungen der Langerhansschen Inseln von Nattern (Natrix natrix L., Natrix tesselata Laurenti). Z. Zellforsch. 90, 519–534 (1968).Google Scholar
  26. Watari, N., Tsukakoshi, N., Homma, Y.: The correlative light and electron microscopy of the islets of Langerhans of some lower vertebrates. Arch. histol. jap. 31, 371–392 (1970).Google Scholar
  27. Yamada, K., Nakamura, M.: An improvement of Dalton's fixative. Technics of electron microscopy, p. 302 (ed. Kanto Branch of Japanese Society of Electron Microscopy. Tokyo: Seibundo-Shinkosha 1970).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Mitusuo Nakamura
    • 1
    • 2
  • Motoyoshi Yokote
    • 1
    • 2
  1. 1.Department of AnatomyNagoya University School of MedicineNagoyaJapan
  2. 2.Freshwater Fisheries Research LaboratoryHino, TokyoJapan

Personalised recommendations