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Über intercelluläre Verbindungen in normaler menschlicher Epidermis

Str. spinosum und Str. granulosum

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Summary

By use of the electron microscope three types of intercellular connections between the cells of normal human epidermis can be demonstrated. These are: the well-known desmosomes, simple desmosomes and nexus.

The fine structure of these intercellular connections is described. The author's findings indicate that the “intermediate dense layer's” (Odland) of desmosomes correspond to the outer leaf of the plasmalemma, and they do not belong to the intercellular space.

The possible role of the nexus in relation to the barrier-function of the epidermis is discussed.

Zusammenfassung

Es konnten elektronenmikroskopisch drei Formen der zwischenzelligen Kontaktaufnahme normaler menschlicher Epidermis festgestellt werden, und zwar neben den bekannten hochstrukturierten Desmosomen auch einfache Desmosomen und Nexus.

Die Struktur der genannten zwischenzelligen Verbindungen wurde beschrieben. Es konnte dabei gezeigt werden, daß die “intermediate dense layers” (Odland) der Desmosomen dem äußeren Blatt des Plasmalemms entsprechen und nicht als dem intercellulären Bereich zugehörig zu betrachten sind.

Die mögliche Rolle des Nexus für die Barrierefunktion der Epidermis wurde ventiliert.

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Literatur

  1. Afzelius, B. A.: Chemical fixatives for electron microscopy. In: Harris, R. J. C.: The interpretation of ultrastructure, S. 1–10. New York und London: Academic Press 1962.

    Google Scholar 

  2. Braun-Falco, O., u. G. Petry: Zur Feinstruktur der Epidermis bei chronischem nummulärem Ekzem. Arch. klin. exp. Derm. 222, 219–241 (1965).

    Google Scholar 

  3. —, u. W. Vogell: Elektronenmikroskopische Untersuchungen zur Dynamik der Akantholyse bei Pemphigus vulgaris. Arch. klin. exp. Derm. 223, 328–346 (1965).

    Google Scholar 

  4. Brody, I.: The ultrastructure of the tonofibrils in the keratinization process of normal human epidermis. J. Ultrastruct. Res. 4, 264–297 (1960).

    Google Scholar 

  5. —: The ultrastructure of the epidermis in Psoriasis vulgaris. J. Ultrastruct. Res. 6, 304–323 (1962).

    Google Scholar 

  6. Dewey, M. M., and L. Barr: A study of the structure and distribution of the nexus. J. Cell Biol. 23, 553–585 (1964).

    Google Scholar 

  7. Ellis, R. A., and W. Montagna: Electron microscopy of the duct and especially the “Cuticular Border” of the eccrine sweat glands in Macaca mulatta. J. biophys. biochem. Cytol. 9, 238–242 (1961).

    Google Scholar 

  8. Farquhar, M. G., and G. E. Palade: Junctional complexes in various epithelia. J. Cell Biol. 17, 375–412 (1963).

    Google Scholar 

  9. Fasske, E., u. H. Themann: Über das Deckepithel der menschlichen Mundschleimhaut. Z. Zellforsch. 49, 447–463 (1959).

    Google Scholar 

  10. Grimley, Ph. M., and G. A. Edwards: The ultrastructure of cardiac desmosomes in the toad and their relationship to the intercalated disc. J. biophys. biochem. Cytol. 8, 305–318 (1960).

    Google Scholar 

  11. Haim, G.: Elektronenmikroskopische Untersuchungen des normalen Epithels der menschlichen Mundschleimhaut, S. 7–52. München: Carl Hanser 1964.

    Google Scholar 

  12. Hama, K.: The fine structure of the demosomes in frog mesothelium. J. biophys. biochem. Cytol. 7, 575–577 (1960).

    Google Scholar 

  13. Hibbs, R. G., and W. H. Clark jr.: Electron microscope studies of the human epidermis. The cell. Boundaries and topography of the Stratum Malpighi. J. biophys. biochem. Cytol. 6, 71–76 (1959).

    Google Scholar 

  14. Horstmann, E., u. A. Knoop: Elektronenmikroskopische Studien an der Epidermis. Z. Zellforsch. 47, 348–362 (1958).

    Google Scholar 

  15. Karrer, H. E.: Interconnections in normal human cervical epithelium. J. biophys. biochem. Cytol. 7, 181–184 (1960).

    Google Scholar 

  16. Lagerholm, B., and A. Frithz: Cellular changes in the psoriatic epidermis. Acta derm.-venereol. (Stockh.) 44, 385–398 (1964).

    Google Scholar 

  17. Locke, M.: The structure of septate desmosomes. J. Cell Biol. 25, 166–169 (1965).

    Google Scholar 

  18. Merker, H.-J.: Das elektronenmikroskopische Bild der Haftstellen (Desmosomen) im Vaginalepithel der Ratte. Berl. Med. 12, 555–558 (1961).

    Google Scholar 

  19. Muir, A. R., and A. Peters: Quintuple-layered membrane junctions at terminal bars between endothelial cells. J. Cell Biol. 12, 443–448 (1962).

    Google Scholar 

  20. Odland, G. F.: The fine structure of the interrelationship of cells in the human epidermis. J. biophys. biochem. Cytol. 4, 529–538 (1958).

    Google Scholar 

  21. Petry, G.: Desmosomen. Dtsch. med. Wschr. 87, 1012–1014 (1962).

    Google Scholar 

  22. —, L. Overbeck u. W. Vogell: Vergleichende elektronen- und lichtmikroskopische Untersuchungen am Vaginalepithel in der Schwangerschaft. Z. Zellforsch. 54, 382–401 (1961).

    Google Scholar 

  23. ———: Sind Desmosomen statische oder temporäre Zellverbindungen? Naturwissenschaften 48, 166–167 (1962).

    Google Scholar 

  24. Porter, K. R.: Observations on the submicroscopic structure of animal epidermis. Anat. Rec. 118, 433 (1954).

    Google Scholar 

  25. Puccinelli, V.: Il desmosoma nell'epithelio cutaneo. G. ital. Derm. 105, 281–294 (1964).

    Google Scholar 

  26. Ranvier, L. A.: Novelles recherches sur le mode d'union des cellules du corps muqueux de Malpighi. Zit. nach G. F. Odland: C.R. Acad. Sci. (Paris) 95, 1374 (1882).

    Google Scholar 

  27. Roth, S. I., and W. H. Clark jr.: Ultrastructural evidence related to the mechanism of keratin synthesis. In: Montagna, W., and W. C. Lobitz jr.: The epidermis. S. 303–337. New York und London: Academic Press 1964.

    Google Scholar 

  28. Selby, C.C.: An electron microscope study of the epidermis of mammalian skin in thin sections. J. biophys. biochem. Cytol. 1, 429–444 (1955).

    Google Scholar 

  29. Snell, R.: An electron microscopic study of keratinization in the epidermal cells of the guinea-pig. Z. Zellforsch. 65, 829–846 (1965).

    Google Scholar 

  30. Swanbeck, G., and N. Thyresson: The role of keratohyalin material in the keratinization process and its importance for the barrier function. Acta derm.-venereol. (Stockh.) 45, 21–25 (1965).

    Google Scholar 

  31. Tamarin, A., and L. M. Sreebny: An analysis of desmosome shape, size, and orientation by the use of histometric and densitometric methods with electron microscopy. J. Cell Biol. 18, 125–134 (1963).

    Google Scholar 

  32. Tandler, B.: Ultrastructure of the human submaxilary gland. J. Ultrastruct. Res. 9, 65–75 (1963).

    Google Scholar 

  33. Vogel, A.: Zum Feinbau der Intercellularbrücken nach Kontrastierung mit Phosphorwolframsäure. 4. Internat. Kongr. f. Elektronenmikroskopie Berlin 1959, Verhandl. Bd. II, Biolog.-med. Teil, S. 286–289. Berlin, Göttingen, Heidelberg: Springer 1960.

    Google Scholar 

  34. Wilgram, G. F., J. B. Coulfield, and E. B. Magdic: An electron microscopic study of acantholysis and dyskeratosis in Pemphigus foliaceus. J. invest. Derm. 43, 287–299 (1964).

    Google Scholar 

  35. Wood, R. L.: Intercellular attachment in the epithelium of hydra as revealed by electron microscopy. J. biophys. biochem. Cytol. 6, 343–351 (1959).

    Google Scholar 

  36. Zelickson, A. S.: Electron microscopy of skin and mucous membrane. Springfield: Ch. C. Thomas 1963.

    Google Scholar 

  37. —, and J. F. Hartmann: An electron microscopic study of human epidermis. J. invest. Derm. 36, 65–72 (1961).

    Google Scholar 

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  1. Dermatologischen Klinik und Poliklinik der Philipps-Universität Marburg/Lahn, Marburg/Lahn, Deutschland

    M. Rupec

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Rupec, M. Über intercelluläre Verbindungen in normaler menschlicher Epidermis. Arch. klin. exp. Derm. 224, 32–41 (1966). https://doi.org/10.1007/BF00522624

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  • DOI: https://doi.org/10.1007/BF00522624

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