Continuous production of glucose-6-phosphate by immobilized Achromobacter butyri cells

  • K. Murata
  • T. Uchida
  • K. Tani
  • J. Kato
  • I. Chibata
Biotechnology

Summary

Whole cells of Achromobacter butyri OUT 8004 having polyphosphate glucokinase activity were immobilized in polyacrylamide gel. The immobilized cells were activated by organic solvents, especially acetone. The immobilization resulted in increased stability of polyphosphate glucokinase. Continuous high yield production of G-6-P from glucose and metaphosphate was performed with an immobilized cell column, which had a half-life of approximately 20 days.

Keywords

Glucose Acetone Immobilization Polyacrylamide Organic Solvent 

Abbreviations

G-6-P

glucose-6-phosphate

G-1-P

glucose-1-phosphate

Cation-S

stearyl trimethyl ammonium chloride

SDS

sodium dodecyl sulfate

Tris

tris(hydroxymethyl)-aminomethane; p-NPP, p-nitrophenyl phosphate

S.V.

space velocity

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References

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • K. Murata
    • 1
  • T. Uchida
    • 1
  • K. Tani
    • 1
  • J. Kato
    • 1
  • I. Chibata
    • 1
  1. 1.Department of Biochemistry, Research Laboratory of Applied BiochemistryTanabe Seiyaku Co., Ltd.OsakaJapan

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