Current Genetics

, Volume 14, Issue 5, pp 461–470 | Cite as

Cloning and sequencing of a cDNA encoding the small subunit precursor of ribulose-1,5-bisphosphate carboxylase from Chlamydomonas moewusii

Evolution of RUBISCO ss polypeptide
  • Claire Simard
  • Claude Lemieux
  • Guy Bellemare
Original Articles


We have isolated and characterized a full-length cDNA clone encoding the precursor of the small subunit (pSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from the green alga, Chlamydomonas moewusii. Comparison with the C. reinhardtii rbcS1 gene sequence reveals that both small subunit (SS) coding regions are 75% homologous and that their predicted mature polypeptide chains are each composed of 140 amino acids. In contrast, their transit peptides appear to be divergent. We also show that transcription of the C. moewusiirbcS gene(s) which generates a 1,230 and a 930 base mRNA species are light-stimulated/or accumulated during the light period of the cell cycle. Finally, the SS polypeptide sequences of fifteen different photosynthetic organisms are compared; this analysis reveals at least five well-conserved polypeptide domains.

Key words

Evolution Sequence comparison RUBISCO Transit peptide 


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  1. Bedbrook JR, Smith SM, Ellis RJ (1980) Nature 287:692–697Google Scholar
  2. Bellemare G, Bartlett SG, Chua NH (1982) J Biol Chem 257:7762–7767Google Scholar
  3. Berry-Lowe SL, McKnight TD, Shah DM, Meagher RB (1982) J Mol Appl Genet 1:483–498Google Scholar
  4. Blair GE, Ellis RJ (1973) Biochim Biophys Acta 319:223–234Google Scholar
  5. Broglie R, Bellemare G, Bartlett SG, Chua NH (1981) Proc Natl Acad Sci USA 78:7304–7308Google Scholar
  6. Broglie R, Coruzzi G, Lamppa G, Keith B, Chua NH (1983a) Bio/Technology 1:55–61Google Scholar
  7. Broglie R, Coruzzi G, Lamppa G, Keith B, Chua NH (1983b) Stadler Symposium, University of Missouri, Columbia 15:59–71Google Scholar
  8. Burnette WN (1981) Anal Biochem 112:195–203Google Scholar
  9. Carmichael GG, McMaster GK (1980) The analysis of nucleic acids in gels using glyoxal and acridine organe. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 65. Academic Press, New York London, pp 380–391Google Scholar
  10. Cashmore AR (1979) Cell 17:383–388Google Scholar
  11. Chua NH, Schmidt GW (1978) Proc Natl Acad Sci USA 75:6110–6114Google Scholar
  12. Coruzzi G, Broglie R, Cashmore A, Chua NH (1983) J Biol Chem 258:1399–1402Google Scholar
  13. Curtis SE, Clegg MT (1984) Mol Biol Evol 1:291–301Google Scholar
  14. Dean C, Van den Elzen P, Tamaoki S, Black N, Dunsmuir V, Bedbrook J (1987) Mol Gen Genet 206:465–474Google Scholar
  15. DeRocher EJ, Ramage RT, Michalowski CB, Bohnert HJ (1987) Nucleic Acids Res 15:6301Google Scholar
  16. Devereux J, Haeberli P, Smithies O (1984) Nucleic Acids Res 12:387–395Google Scholar
  17. Dobberstein B, Blobel G, Chua NH (1977) Proc Natl Acad Sci USA 74:1082–1085Google Scholar
  18. Dretzen G, Bellard M, Sassone-Corsi P, Chambon P (1981) Anal Biochem 112:295–298Google Scholar
  19. Dunsmuir P, Smith S, Bedbrook J (1983) Nucleic Acids Res 11:4177–4183Google Scholar
  20. Ellis RJ (1979) Trends Biochem Sci 4:241–244Google Scholar
  21. Fitzgerald M, Shenk T (1981) Cell 24:251–260Google Scholar
  22. Goldschmidt-Clermont M, Rahire M (1986) J Mol Biol 191:421–432Google Scholar
  23. Gooding LR, Roy H, Jagendorf AT (1973) Arch Biochem Biophys 159:324–335Google Scholar
  24. Gowans CS (1960) Z Vererbungsl 91:63–73Google Scholar
  25. Herrin D, Michaels A (1984) Plant Mol Biol Rep 2:24–28Google Scholar
  26. Highfield PE, Ellis RJ (1978) Nature 271:420–424Google Scholar
  27. Kahana ZE, Edelman M (1982) Isolation and purification of RuBP carboxylase by sucrose gradient centrifugation, DEAE cellulose chromatography and immunoaffinity columns. In: Edelman M, Hallick R, Chua NH (eds) Methods in chloroplast molecular biology, chap 63. Elsevier Biomedical Press, pp 793–802Google Scholar
  28. Karlin-Newmann GA, Kohorn BD, Thornber JP, Tobin EM (1985) J Mol Appl Gen 3:45–61Google Scholar
  29. Lebrun M, Waksman G, Freyssinet G (1987) Nucleic Acids Res 15:4360Google Scholar
  30. Lemieux B, Turmel M, Lemieux C (1985) BioSystems 18:293–298Google Scholar
  31. Lewin B (1983) Genes. Library of Congress Cataloging in Publication Data, USA, pp 715Google Scholar
  32. Lorimer GH (1981) Annu Rev Plant Physiol 32:349–383Google Scholar
  33. Mandel M, Higa A (1970) J Mol Biol 53:159–162Google Scholar
  34. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
  35. Martin PG (1979) Aust J Plant Physiol 6:401–408Google Scholar
  36. Maxam A, Gilbert W (1980) Sequencing end-labeled DNA with base specific chemical cleavages. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 65. Academic Press, New York, pp 499–560Google Scholar
  37. McKnight TD, Alexander DC, Babcock MS, Simpson RB (1986) Gene 48:23–32Google Scholar
  38. Mishkind ML, Schmidt GW (1983) Plant Physiol 72:847–854Google Scholar
  39. Miziorko HM, Lorimer GH (1983) Annu Rev Biochem 52:507–535Google Scholar
  40. Muller KD, Salnikow J, Vater J (1983) Biochim Biophys Acta 742:78–83Google Scholar
  41. Nierzwicki-Bauer SA, Curtis SE, Haselkorn R (1984) Proc Natl Acad Sci USA 81:5961–5965Google Scholar
  42. Okayama H, Berg P (1982) Mol Cell Biol 2:161–170Google Scholar
  43. Piccioni R, Bellemare G, Chua NH (1982) Methods of polyacrylamide gel electrophoresis in the analysis and preparation of plant polypeptides. In: Edelman M, Hallick R, Chua NH (eds) Methods in chloroplast molecular biology, chap 80. Elsevier Biomedical Press, pp 985–1014Google Scholar
  44. Pinck L, Fleck J, Pinck M, Hadidane R, Hirth L (1983) FEBS Lett 154:145–148Google Scholar
  45. Rochaix JD, Malnoë P (1982) Use of DNA-RNA hybridizations for locating chloroplast genes and for estimating the size and abundance of chloroplast DNA transcripts. In: Edelman M, Hallick R, Chua NH (eds) Methods in chloroplast molecular biology, chap 36. Elsevier Biomedical Press, pp 477–489Google Scholar
  46. Rochaix JD, van Dillewijn J (1982) Nature 296:70–72Google Scholar
  47. Sailland A, Amiri I, Freyssinet G (1986) Plant Mol Biol 7:213–218Google Scholar
  48. Schmidt GW, Devillers-Thiery A, Desruisseaux H, Blobel G, Chua NH (1979) J Cell Biol 83:615–622Google Scholar
  49. Schmidt RJ, Myers AM, Gillham NW, Boynton JE (1984) Mol Biol Evol 1:317–334Google Scholar
  50. Shinozaki K, Sugiura M (1983) Nucleic Acids Res 11:6957–6964Google Scholar
  51. Silflow CD, Chisholm RX, Conner TW, Ranun LPW (1985) Mol Cell Biol 5:2389–2398Google Scholar
  52. Simard C (1986) Clonage et séquengage de l'ADN complémentaire codant le précurseur de la petite sous-unité de la RUBISCO de Chlamydomonas moewusii. These de Doctorat, Université Laval, QuébecGoogle Scholar
  53. Smeekens S, van Oosten J, de Groot M, Weisbreek P (1986) Plant Mol Biol 7:433–440Google Scholar
  54. Smith MA (1984) Mol Cell Biochem 63:149–156Google Scholar
  55. Stiekema WJ, Wimpee CF, Tobin EM (1983) Nucleic Acids Res 11:8051–8061Google Scholar
  56. Sugita M, Manzara T, Pichersky E, Cashmore A, Gruissem W (1987) Mol Gen Genet 209:247–256Google Scholar
  57. Thomas PS (1980) Proc Natl Acad Sci USA 77:5201–5205Google Scholar
  58. Vieira J, Messing J (1982) Gene 19:259–268Google Scholar
  59. Waksman G, Freyssinet G (1987) Nucleic Acids Res 15:1328Google Scholar
  60. Whiteway MS, Lee RW (1977) Mol Gen Genet 157:11–15Google Scholar
  61. Whitfeld PR, Bottomley W (1983) Annu Rev Plant Physiol 34:279–310Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Claire Simard
    • 1
  • Claude Lemieux
    • 1
  • Guy Bellemare
    • 1
  1. 1.Département de biochimie, Faculté des sciences et de génieUniversité LavalQuébecCanada

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