Current Genetics

, Volume 14, Issue 5, pp 461–470 | Cite as

Cloning and sequencing of a cDNA encoding the small subunit precursor of ribulose-1,5-bisphosphate carboxylase from Chlamydomonas moewusii

Evolution of RUBISCO ss polypeptide
  • Claire Simard
  • Claude Lemieux
  • Guy Bellemare
Original Articles

Summary

We have isolated and characterized a full-length cDNA clone encoding the precursor of the small subunit (pSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from the green alga, Chlamydomonas moewusii. Comparison with the C. reinhardtii rbcS1 gene sequence reveals that both small subunit (SS) coding regions are 75% homologous and that their predicted mature polypeptide chains are each composed of 140 amino acids. In contrast, their transit peptides appear to be divergent. We also show that transcription of the C. moewusiirbcS gene(s) which generates a 1,230 and a 930 base mRNA species are light-stimulated/or accumulated during the light period of the cell cycle. Finally, the SS polypeptide sequences of fifteen different photosynthetic organisms are compared; this analysis reveals at least five well-conserved polypeptide domains.

Key words

Evolution Sequence comparison RUBISCO Transit peptide 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Claire Simard
    • 1
  • Claude Lemieux
    • 1
  • Guy Bellemare
    • 1
  1. 1.Département de biochimie, Faculté des sciences et de génieUniversité LavalQuébecCanada

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