Summary
We have constructed a series of deletion plasmids which contain the Aspergillus nidulans argB gene for ornithine carbamoyltransferase (OTC). These deletions comprise the 5′ upstream sequence of the argB gene. The pro− arg− strain of A. nidulans was transformed with the above plasmids. Several arg+ transformants of integration types I and II, obtained using each of the deletion plasmids, were studied, and their ability to de-repress OTC level by proline starvation was compared. It was concluded that nucleotides located between −150 and −50 by upstream of the argB gene are significant for its cross-pathway regulation. This regulatory region contains three copies of the TGACTC hexanucleotide which is a cis-acting regulatory sequence of general amino acid control in yeast.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- bp :
-
base pairs
- kb :
-
103 base pairs
- OTC :
-
ornithine carbamoyltransferase
- ORF :
-
open reading frame
References
Arndt K, Fink GR (1986) Proc Natl Acad Sci USA 83:8516–8520
Beachem IR, Schweitzer BW, Warrick HM, Carbon J (1984) Gene 29:271–279
Beggs JD (1978) Nature 275:104–109
Berse B, Dmochowska A, Skrzypek M, Wěgleński P, Bates MA, Weiss RL (1983) Gene 25:109–117
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1525
Bradford M (1976) Anal Biochem 72:248–254
Carsiostis M, Jones RF (1974) J Bacteriol 119:889–892
Carsiostis M, Jones RF, Wesseling AC (1974) J Bacteriol 119:893–898
Crabeel M, Huygen R, Verschueren K, Messenguy F, Tinel K, Cunin R, Glansdorf N (1985) Mol Cell Biol 5:3139–3148
Cybis J, Piotrowska M, Wěgleński P (1970) Bull Acad Pol Sci Ser Sci Biol 18:669–672
Delforge J, Messenguy F, Wiame JM (1975) Eur J Biochem 57:231–239
Denhardt D (1966) Biochem Biophys Res Commun 23:641–646
Dmochowska A, Berse B, Kowalska I, Skrzypek M, Wěgleński P (1986) J Gen Microbiol 132:1729–1737
Donahue TF, Daves RS, Lucchini G, Fink GR (1983) Cell 32:89–98
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Hinnebusch AG (1986) CRC Crit Rev Biochem 21:277–317
Hinnebusch AG, Fink GR (1983a) J Biol Chem 258:5238–5247
Hinnebusch AG, Fink GR (1983b) Proc Natl Acad Sci USA 80:5374–5378
Hinnebusch AG, Lucchini G, Fink GR (1985) Proc Natl Acad Sci USA 82:498–502
Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
Hope IA, Struhl K (1985) Cell 43:177–188
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Piotrowska M (1980) J Gen Microbiol 116:335–339
Pontecorvo C, Roper JA, Hemmons LM, MacDonald KD, Bufton AWJ (1953) Adv Genet 5:141–238
Schürch A, Miozzari J, Hütter R (1974) J Bacteriol 117:1131–1140
Soberon X, Covarrubias L, Bolivar F (1980) Gene 9:287–305
Southern EM (1975) J Mol Biol 98:503–517
Struhl K (1982) Nature 300:284–287
Upshall A, Gilbert T, Saari G, O'Hara PJ, Wěgleński P, Berse B, Miller K, Timberlake WE (1986) Mol Gen Genet 204:349–354
Werner M, Feller A, Pierard A (1985) Eur J Biochem 146:371–381
Yelton MM, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474
Zalkin H, Yanofsky C (1982) J Biol Chem 257:1491–1500
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Goc, A., Wěgleńiski, P. Regulatory region of the Aspergillus nidulans argB gene. Curr Genet 14, 425–429 (1988). https://doi.org/10.1007/BF00521264
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00521264