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Elektronenmikroskopische Untersuchungen zum Problem des Stoffaustausches zwischen Mutter und Keim bei Rattenembryonen des Tages 7–10

Electron microscopic observations on the barriers between mother and embryos in pregnant rats of days 7–10

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Summary

Electron microscope studies of rat embryos were performed from the 7th to 10th days of pregnancy. The single-layered visceral yolk entoderm is characterized by a marked endocytotic activity. The cells of the parietal layer lie separately adjacent to the Reichert's membrane and they contain mainly rough endoplasmic reticulum. The Reichert's membrane consists of a loose network of fine filaments. In the antimesometrial and lateral sites only few trophoblast cells are seen to be attached to the membrane. Between them we find large gaps. With the exception of the mesometrial pole, the embryo is surrounded by a periembryonal sinus, 10–50 μ wide, which contains maternal blood. This sinus space is sealed tightly (zonulae occludentes) by trophoblast and decidual cells. From these findings it was concluded: 1. Reichert's membrane is formed from the parietal jolk sac entoderm. 2. The porous Reichert's membrane permits the maternal plasma to reach freely the visceral jolk sac entoderm. This membrane is the only continuous layer between blood and egg cylinder. 3. By the endocytotic and lysosomal system, the visceral jolk sac entoderm absorbs the plasma coming from the mother. This plasma is degraded and used for the growing embryo. 4. From the immunobiological point of view the antibodies from the mother can reach the embryo freely, 5. The embryonal antigens cannot reach the maternal circulation because of the presence of a decidual barrier, 6. The perimebryonal sinuses are blind sacs into which the maternal blood can flood but it cannot return.

Zusammenfassung

Rattenkeime des Schwangerschaftstages 7–10 wurden elektronenmikroskopisch untersucht. Das viscerale einschichtige Dottersackentoderm zeichnet sich durch eine ausgeprägte endocytotische Aktivität aus. Die Zellen des parietalen Blattes dagegen liegen isoliert der Reichertschen Membran innen an und enthalten vorwiegend rauhes endoplasmatisches Reticulum. Die Reichertsche Membran besteht aus einem lockeren Maschengitter feiner Filamente. Im antimesometrialen und lateralen Bereich haften nur wenige Trophoblastenzellen dieser Membran von außen an, dazwischen befinden sich weite Lücken. Zum mütterlichen Gewebe hin wird der Keim mit Ausnahme des mesometrialen Poles von einem bis zu 50 μ breiten periembryonalen Sinus umgeben, der mütterliches Blut enthält. Nach außen wird dieser Raum durch Trophoblasten- und Deciduazellen abgedichtet. Aus diesen Befunden wird geschlossen: 1. Die Reichertsche Membran wird vom parietalen Dottersack-Entoderm gebildet. 2. Mütterliches Blutplasma kann frei das viscerale Dottersack-Entoderm erreichen, da als einzige kontinuierliche Schicht zwischen Blut und Träger nur die weitmaschige Reichertsche Membran vorhanden ist. 3. Durch das endocytotische und lysosomale System des visceralen Dottersackentoderms wird das anströmende mütterliche Plasma aufgenommen, abgebaut und für den wachsenden Keim nutzbar gemacht. 4. Immunbiologisch betrachtet können Antikörper der Mutter den Keim erreichen. 5. Embryonale Antigene dagegen erreichen durch die Errichtung einer Deciduabarriere nicht den mütterlichen Kreislauf. 6. Der periembryonale Sinus stellt einen Blindsack dar, in den zwar mütterliches Blut einströmen kann, ein Ausstrom aber nicht mehr möglich ist.

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  1. H. Villegas (Stipendiat des Senats der Stadt Berlin)

    Present address: Instituto Mexicano Del Seguro Social Hospital de Gineco-Obstetricia No. Uno Gabriel Mancera 222, Mexico 12, D.F.

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  1. II. Anatomisches Institut der Freien Universität Berlin, Berlin, Deutschland

    H. -J. Merker & H. Villegas (Stipendiat des Senats der Stadt Berlin)

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Durchgeführt mit Unterstützung durch die DFG im Rahmen des Sonderforschungsbereiches „Embryonalpharmakologie”.

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Merker, H.J., Villegas, H. Elektronenmikroskopische Untersuchungen zum Problem des Stoffaustausches zwischen Mutter und Keim bei Rattenembryonen des Tages 7–10. Z. Anat. Entwickl. Gesch. 131, 325–346 (1970). https://doi.org/10.1007/BF00519974

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