Some characteristics of chick embryo sympathetic ganglion fragments in a simple culture system with and without nerve growth factor
A method is described for the culture of numbers of fragments of chick embryo sympathetic ganglia in similar conditions.
A comparison is made on a morphological basis, between growth of ganglion fragments with and without added NGF.
It is concluded that in this system, 4U NGF/ml NGM has a favourable influence on the maintenance, though not on the initial development, of such cultures, and is optimal for the production by simple techniques of a high yield of comparable cultures containing adrenergic nerve.
Key wordsTissue culture Chick embryo Sympathetic ganglia Nerve growth factor
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