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Some characteristics of chick embryo sympathetic ganglion fragments in a simple culture system with and without nerve growth factor

  • D. R. Mottram
  • R. Presley
  • J. D. Lever
  • C. Ivens
Article

Summary

  1. 1.

    A method is described for the culture of numbers of fragments of chick embryo sympathetic ganglia in similar conditions.

     
  2. 2.

    A comparison is made on a morphological basis, between growth of ganglion fragments with and without added NGF.

     
  3. 3.

    It is concluded that in this system, 4U NGF/ml NGM has a favourable influence on the maintenance, though not on the initial development, of such cultures, and is optimal for the production by simple techniques of a high yield of comparable cultures containing adrenergic nerve.

     

Key words

Tissue culture Chick embryo Sympathetic ganglia Nerve growth factor 

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References

  1. Brown, W. T., Larrabee, M. G., Parlow, L. M.: Unpublished, cited in Jacobson, M.: Developmental neurobiology, p. 217. New York: Holt, Rinehart, Winstone 1970.Google Scholar
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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • D. R. Mottram
    • 1
  • R. Presley
    • 1
  • J. D. Lever
    • 1
  • C. Ivens
    • 1
  1. 1.Department of AnatomyUniversity CollegeCardiff

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