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Evaluation of the antigen from chlamydospores of Candida albicans in the serodiagnosis of candidiasis

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Abstract

Antigens have been prepared from the chlamydospores and blastospores of Candida albicans and their precipitin patterns were analysed by two-dimensional immunoelectrophoresis using specific antisera.

The two antigens were used in routine serological tests of patients suffering from candidiasis. On double-diffusion tests for the detection of circulating antibodies of Candida albicans, the antigen from chlamydospores displays precipitin lines that differ in number and intensity from those obtained with the antigen from blastospores. The results are briefly discussed in the framework of C. albicans antigen standardization.

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References

  1. Axelsen, N. H., Kirkpatrick, C. H. & Buckley, R. H., 1974. Precipitins to Candida albicans in chronic mucocutaneous candidiasis studied by crossed immunoelectrophoresis with intermediate gel. Correlation with clinical and immunological findings. Clin. Exp. Immunol. 17: 385–394.

    Google Scholar 

  2. Chew, W. H. & Theus, T. L., 1967. Candida precipitin. J. Immunol. 98: 220–224.

    Google Scholar 

  3. Evans, E. G. V., 1976. Serology of fungal infection and farmer's lung disease. A Laboratory Manual. Leeds University Printing Service. British Soc. Mycopath. Ed. Evans.

  4. Evans, E. G. V. & Forster, R. A., 1976. Antibodies to Candida after operations on the heart. J. Med. Microb. 9: 303–308.

    Google Scholar 

  5. Evans, E. G. V., Richardson, M. D., Odds, F. C. & Holland, K. T., 1973. Relevance of antigenicity of Candida albicans growth phases to diagnosis of systemic candidiasis. Brit. Med. J. 4: 86–87.

    Google Scholar 

  6. Fragner, P. & Kvicera, J., 1968. Candida albicans netvorici chlamydospory. Ceska Mykol. 22: 112–113.

    Google Scholar 

  7. Heinman, H. S., Yunis, E. J., Siemienski, J. & Braude, A. I., 1961. Chlamydospores and dimorphism in Candida albicans endocarditis. Archs. intern. Med. 108: 126–133.

    Google Scholar 

  8. Munoz, M., Estes, G., Kilpatrick, M., Di Salvo, A. & Virella, G., 1980. Purification of cytoplasmic antigens from the mycelial phase of Candida albicans: possible advantages of its use in Candida serology. Mycopathologia 72: 47–53.

    Google Scholar 

  9. Odds, F. C., Evans, E. G. V., Taylor, M. A. R. & Wales, J. K., 1978. Prevalence of pathogenic yeasts and humoral antibodies to Candida in diabetic patients. J. Clin. Pathol. 31: 840–844.

    Google Scholar 

  10. Ouchterlony, O., 1965. Diffusion in gel methods for immunological analysis. In: Progress in Allergy, S. Karger, Basel. II, pp. 30–154.

    Google Scholar 

  11. Poulain, D., Desseaux, G. & Vernes, A., 1978. Variation de la structure antigénique pariétale des blastospores de Candida albicans. II. Appréciation de la richesse de souches clonées en antigénes P. Influence du milieu de culture. Bull. Soc. Myc. Med. 7: 37–44.

    Google Scholar 

  12. Poulain, D., Vernes, A. & Biguet, J., 1980. Variation of the antigenic structure of Candida albicans blastospores cell wall. Practical repercussions. Medical Mycol. Zbl. Bakt. Suppl. 8: 147–156. Eds. Preusser.

    Google Scholar 

  13. Poulain, D., Vernes, A. & Frutt, J., 1980. Variation de la structure antigenique parietale de Candida albicans mise en evidence au niveau des blastospores d'un antigene P dependant de leur origine. Sabouraudia 18: 61–68.

    Google Scholar 

  14. Strippoli, V. & Simonetti, N., 1971. Separation and characterization of morphological elements of Candida albicans by Urovison density gradient. Mycopath. Mycol. appl. 45: 77–84.

    Google Scholar 

  15. Syverson, R. E., Buckley, H. R. & Campbell, C. C., 1975. Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans. Infect. Immun. 12: 1184–1188.

    Google Scholar 

  16. Umenau, T. & Chiba, H., 1977. Evaluation of the antigen specific to the mycelial phase of Candida albicans in the serodiagnosis of candidiasis. Tohoku J. Exp. Med. 123: 395–396.

    Google Scholar 

  17. Warnock, D. W., Speller, D. C. E., Finan, P. J., Wellacott, K. D. & Phillips, M. N., 1979. Antibodies to Candida species after operations on the large intestine: observations on the association with oral and faecal yeast colonization. Sabouraudia 17: 405–414.

    Google Scholar 

  18. Weyman-Rzucidlo, D., 1965. Histochemical and immunological study of Candida albicans chlamydospores. I. Histochemical properties of C. albicans chlamydospores (double staining method of chlamydospores). Act. Microbiol. pol. 14: 237–245.

    Google Scholar 

  19. Weyman-Rzucidlo, D., 1965. Histochemical and immunological study of Candida albicans chlamydospores. II. Immunofluorescent study of C. albicans chlamydospores. Acta Microbiol. pol. 14: 247–250.

    Google Scholar 

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Caretta, G., Del Frate, G. & Guglielminetti, M. Evaluation of the antigen from chlamydospores of Candida albicans in the serodiagnosis of candidiasis. Mycopathologia 77, 159–163 (1982). https://doi.org/10.1007/BF00518801

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