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Immunohistochemical detection of ganglia in the rat stomach serosa, containing neurons immunoreactive for gastrin-releasing peptide and vasoactive intestinal peptide

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Summary

Ganglia, not previously described, were identified in the rat stomach serosa along the minor curvature. The ganglia consisted of varying number of cell bodies lying in clusters along or within nerve bundles. The ganglia were shown to contain GRP and VIP immunoreactive nerve fibers and cell bodies and also some NPY immunoreactive fibers, whereas they were devoid of somatostatin immunoreactivity. Nerve ligation experiments indicated that the ganglia are intrinsic to the stomach.

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References

  • Baldissera FGA, Holst JJ, Jensen SL, Krarup T (1985) Distribution and molecular forms of peptides containing somatostatin immunodeterminants in extracts from the enteric gastrointestinal tract in man and pig. Biochim Biophys Acta 838:132–143

    Google Scholar 

  • Buffa R, Solovieva I, Fiocca R, Giorgino S, Rindi G, Solcia E, Moscizuchi T, Yanaihara C, Yanaihara N (1982) Localization of bombesin and GRP (gastrin releasing peptide) sequences in gut nerves or endocrine cells. Histochemistry 76:457–467

    Google Scholar 

  • Einarson L (1951) On the theory of gallocyanin-chromalum staining and its application for quantitative estimation of basophilia. A selective staining of exquisite progressivity. Acta Pathol Microbiol Immunol Scand 28:82

    Google Scholar 

  • Ekblad E, Ekelund M, Graffner H, Håkoson R, Sundler F (1985) Peptide-containing nerve fibers in the stomach wall of rat and mouse. Gastroenterology 89:73–85

    Google Scholar 

  • Fuxe K, Hökfelt T, Said DI, Mutt V (1977) Vasoactive intestinal polypeptide and the nervous system: Immunohistochemical evidence for localization in central and periferal neurons, particularly intercortical neurons of the cerrebral cortex. Neurosci Lett 5:241–246

    Google Scholar 

  • Iwanaga T (1983) Gastrin-releasing peptide (GRP)/bombesin-like immunoreactivity in the neurons and paraneurons of the gut and lung. Biomed Res 4:93–104

    Google Scholar 

  • Knuthsen S, Holst JJ, Knigge U, Olesen M, Nielsen OV (1984) Radioimmunosassay, Pharmacokinetics, and neuronal release of gastrin-releasing peptide in anesthetized pigs. Gastroenterology 87:372–378

    Google Scholar 

  • Knuthsen S, Holst JJ, Baldissera FGA, Skak-Nielsen T, Poulsen SS, Jensen SL, Nielsen OV (1987) Gastrin-releasing peptide in the porcine pancreas. Gastroenterology (in press)

  • Larsson L-I (1981) Peptide immunocytochemistry. In: Graumann W, Loidja Z, Pearse AGE, Schiebler TH (eds) Progress in histochemistry and cytochemistry 4, vol 13. Gustav Fisher, Stuttgart, New York, pp 69–70

    Google Scholar 

  • Larsson L-I, Fahrenkrug J, Schaffalitzky de Muckadell O, Sundler F, Håkonson R, Rehfeld JF (1976) Localization of vasoactive intestinal peptide (VIP) to central and peripheral neurons. Proc Natl Acad Sci USA 73:3197–3200

    Google Scholar 

  • Moghimzadeh E, Ekman R, Håkonsson R, Yanaihara N, Sundler F (1983) Neuronal gastrin-releasing peptide in the mammalian gut an pancreas. Neuroscience 10:553–563

    Google Scholar 

  • Schultzberg M, Dalsgaard C-J (1983) Enteric origin of bombesin immunoreactive fibers in the rat coeliac-superior mensenteric ganglion. Brain Res 269:190–195

    Google Scholar 

  • Sternberger L (1974) Immunocytochemistry. Prentice Hall, Englewood Cliffs, NJ, pp 129–171

    Google Scholar 

  • Torre de la JC, Surgeon JW (1976) A methodological approach to rapid and sensitive monoamine histofluorescense using a modified glyocylic acid technique: The SPG method. Histochemistry 49:81–93

    Google Scholar 

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Skak-Nielsen, T., Poulsen, S.S. & Holst, J.J. Immunohistochemical detection of ganglia in the rat stomach serosa, containing neurons immunoreactive for gastrin-releasing peptide and vasoactive intestinal peptide. Histochemistry 87, 47–52 (1987). https://doi.org/10.1007/BF00518723

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  • DOI: https://doi.org/10.1007/BF00518723

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