Summary
Birefringence and fluorochrome lipid staining with benzpyrene are demonstrated as simple morphological methods to reveal the presence or absence of surfactant lipids in human newborn lung tissue. Lack of lipid birefringence proves to be an associated finding in the lungs of premature infants with hyaline membrane disease, indicating the possible pathogenetical importance of surfactant deficiency.
References
Auld, P.A.M.: Diseases of the respiratory system. In: Diseases of the Fetus and Infant, p. 345–385. Behrman, R.E. Saint Louis: Mosby Comp. 1973
Balis, J.U., Delivora, M., Conen, P.E.: Maturation of postnatal human lung and the idiopathic respiratory distress syndrome. Lab. Invest. 15, 530–546 (1966)
Berg, N.O.: A histological study of masked lipids. Acta Pathol. Microbiol. Scand. (Suppl.) 40, 192 (1951)
Gluck, L., Kulovich Marie, V.: Fetal lung development: Current concepts. Pediatr. Clin. North Am. 20, 367–380 (1973)
Németh, Á.: Demonstration of the pulmonary alveolar lipid membrane structures and granular pneumocytes by polarization and fluorescence microscopy. Acta Histochem. (Jena) 51, 164–171 (1974)
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Németh, Á. Polarization and fluorescent microscopy —Simple methods for demonstrating pulmonary surfactant lipids. Histochemistry 59, 149–151 (1978). https://doi.org/10.1007/BF00518510
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DOI: https://doi.org/10.1007/BF00518510