Summary
After labelling with 14C-thymidine, frozen sections or paraffin sections of the brain of adult mice or rats were first stained by metallic impregnation and then coated with chrome alum gelatine and with an emulsion layer of about 10 μm. On the autoradiographs 14C-tracks are readily recognized above labelled astrocytes or oligodendrocytes, and these can be well discriminated, if the sections are processed by the silver carbonate method of Rio-Hortega. In contrast, no labelling is obtained, if the gold chloride sublimate method of Cajal is applied.
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Baserga, R., Nemeroff, K.: Two-emulsion radioautography. J. Histochem. Cytochem. 10, 628–635 (1962)
Harris, E.B., Hoelzer, D.: DNA synthesis time in leukaemic cells as measured by the double labelling and the percent labelled mitoses methods. Cell Tissue Kinet 4, 433–441 (1971a)
Harris, E.B., Hoelzer, D.: An evaluation of various double labelling and autoradiographic techniques for the measurement of DNA synthesis time in leukaemic cells. J. Microsc. 96, 205–217 (1971b)
Harris, E.B., Kunik, S.D., Kibler, R.: The measurement of DNA synthesis time in bone marrow cells of germ-free and conventional mice by double labelling technique. Radioakt. Isotop. Klin. Forsch. 8, 291–302 (1968)
Hommes, O.R., Leblond, C.P.: Mitotic division of neuroglia in the normal adult rat. J. Comp. Neurol. 129. 269–278 (1967)
Jaros, G.G., Sensenbrenner, M., Wittendorp, E., Rechenmann, R. V., Mandel, P.: Etude traceautoradiographique de la prolifération des cellules nerveuses en culture. C. R. Acad. Sci. (Paris) 279, 1101–1104 (1974)
Kesse-Elias, M., Harris, E.B., Gyftaki, E.: In-vitro study of DNA-synthesis time and cell-cycle time in erythrocyte precursors of normal and Thalassaemic subjects, using a 3H- and 14C-thymidine double labelling technique. Acta Haematol. 38, 170–177 (1967)
Königsmark, B.W., Sidman, R.L.: Origin of brain macrophages in the mouse. J. Neuropathol. Exp. Neurol. 22, 643–676 (1963)
Königsmark, B.W., Sidman, R.L.: Response of astrocytes to brain injury. J. Neuropathol. Exp. Neurol. 24, 142–144 (1965)
Kopriwa, B.M., Leblond, C.P.: Improvements in the coating technique of radioautography. J. Histochem. Cytochem. 10, 269–284 (1962)
Korr, H.: Autoradiographische Untersuchungen zur Proliferation verschiedener Zellelemente im Gehirn von Nagern während der prä- und postnatalen Ontogenese. Habilitationsschrift, University of Würzburg 1978
Korr, H., Schultze, B., Maurer, W.: Autoradiographic investigations of glial proliferation in the brain of adult mice. II. Cycle time and mode of proliferation of neuroglia and endothelial cells. J. Comp. Neurol. 160, 477–490 (1975)
Ling, E.A., Paterson, J.A., Privat, A., Mori, S., Leblond, C.P.: Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain of young rats. J. Comp. Neurol. 149, 43–72 (1973)
Mori, S., Leblond, C.P.: Identification of microglia in light and electron microscopy. J. Comp. Neurol. 135, 57–80 (1969a)
Mori, S., Leblond, C.P.: Electron microscopic features and proliferation of astrocytes in the corpus callosum of the rat. J. Comp. Neurol. 137, 197–226 (1969b)
Mori, S., Leblond, C.P.: Electron microscopical identification of three classes of oligodendrocytes and a preliminary study of their proliferative activity in the corpus callosum of young rats. J. Comp. Neurol. 139, 1–30 (1970)
Murray, H.M., Walker, B.E.: Comparative study of astrocytes and mononuclear leukocytes reacting to brain trauma in mice. Exp. Neurol. 41, 290–302 (1973)
Naoumenko, J., Feigin, I.: A modification for paraffin sections of silver carbonate impregnation for microglia. Acta Neuropathol. (Berl.) 2, 402–406 (1963)
Ramon y Cajal, S.: Contribucion al conocimiento de la neuroglia del cerebro humano. Trab. Lab. Invest. Biol. Univ. Madr. 11, 225–315 (1913)
Rio-Hortega, P. del: El tercer elemento de los entros nervosos. Bol. Soc. Espan. Biol. 9, 68–83 (1919)
Romeis, B.: Mikroskopische Technik. 15 verb. Aufl. München: Oldenbourg 1948
Schultze, B., Hörning, N., Maurer, W.: Blut-Hirn-Schranke und Planzentar-Schranke für 3H-Thymidin und 3H-Cytidin bei der Maus. (Untersuchungen mit Ganzkörper-Autoradiographie.) Z. Naturforsch. 27b, 554–558 (1972)
Schultze, B., Maurer, W., Hagenbusch, H.: A two emulsion autoradiographic technique and the discrimination of the three different types of labelling after double labelling with 3H- and 14C-thymidine. Cell Tissue Kinet. 9, 245–255 (1976)
Sidmann, R.L.: Autoradiographic methods and principles for study of the nervous system with thymidine-H3. In: Contemporary research methods in neuroanatomy Nauta, W.J.H., Ebbesson, S.O.E. (eds.), p. 252–274, Berlin, Heidelberg, New York: Springer 1970
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Korr, H. Combination of metallic impregnation and autoradiography of brain sections. Histochemistry 59, 111–116 (1978). https://doi.org/10.1007/BF00518506
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DOI: https://doi.org/10.1007/BF00518506