Advertisement

Lichtmikroskopische Dünnschnitt-Technik an der Haut

  • C. Orfanos
  • I. Thiele
Article

Zusammenfassung

Es wird über die Anwendungsmöglichkeit der elektronen mikroskopischen Einbettungs- und Dünnschnitt-Technik für lichtmikroskopische Untersuchungen an Hautschnitten (2,0–3,0 μm) berichtet.

Summary

Thin sections (2–3 μm) can easily be obtained from skin fixed in osmium and embedded in Polyester (Vestopal). Such sections can also be stained by several histological and histochemical routine procedures for light microscopical observations (Table 2).

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Cardno, S. S., and J. W. Steiner: Improvement of staining techniques for thin sections of epoxy-embedded tissue. Amer. J. clin. Path. 43, 1 (1965).Google Scholar
  2. Cathey, W.: A plastic embedding medium for thin sectioning in light microscopy. Stain Technol. 38, 213–216 (1963).Google Scholar
  3. Caulfield, J. B., and G. F. Wilgram: An electron-microscope study of dyskeratosis and acantholysis in Darier's disease. J. invest. Derm. 41, 57–65 (1963).Google Scholar
  4. Gautier, A.: Technique de coloration histologique de tissus dans des polyesters. Experientia (Basel) 16, 124 (1960).Google Scholar
  5. Harven, E. de: A propos de l'application de certaines colorations histologiques dans les tissus fixés par l'acide osmique tamponné et inclus dans le méthacrylate de butyle. C. R. Soc. Biol. (Paris) 110, 63–65 (1956).Google Scholar
  6. Jennings, B. M., M. G. Farquar, and H. D. Moon: Staining methods for osmiummethacrylate sections. Amer. J. Path. 35, 991–997 (1959).Google Scholar
  7. Lane, B. P., and D. L. Europa: Differential staining of ultrathin sections of Eponembedded tissues for light microscopy. J. Histochem. Cytochem. 13, 579–582 (1965).Google Scholar
  8. Marinozzi, V.: Techniques nouvelles de coloration de tissus inclus dans des matières plastiques pont l'étude au microscope optique à haute resolution. Z. wiss. Mikr. 65, 219–230 (1963).Google Scholar
  9. Müller-Seifert: Taschenbuch der medizinischen klinischen Diagnostik. Hrsg. H. v. Kress, München: Bergmann 1962.Google Scholar
  10. Richardson, K. C., L. Jarett, and E. H. Finke: Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol. 35, 313 (1960).Google Scholar
  11. Romeis, B.: Mikroskopische Technik. München: R. Oldenbourg 1948.Google Scholar
  12. Runge, J., R. L. Vernier, and J. F. Hartmann: A staining method for sections of osmium-fixed, methacrylate-embedded tissue. J. biophys. biochem. Cytol. 4, 327–328 (1958).Google Scholar
  13. Schwalbach, G., K. G. Lickfeld, and H. Hoffmeister: Differentiated staining of osmium tetroxide-fixed, Vestopal W-embedded tissue in thin sections. Stain Technol. 38, 15–21 (1963).Google Scholar
  14. Thoenes, W.: Giemsa-Färbung an Geweben nach Einbettung in Polyester (“Vestopal”) und Methacrylat. Z. wiss. Mikr. 64, 406–413 (1960).Google Scholar
  15. Trump, B. F., E. A. Schmuckler, and E. P. Benditt: A method for staining epoxy sections for light microscopy. J. Ultrastruct. Res. 5, 343–348 (1961).Google Scholar
  16. Winkelstein, J., M. G. Menefee, and A. Bell: Basic fuchsin as a stain for osmiumfixed, epon-embedded tissue. Stain Technol. 38, 202–204 (1963).Google Scholar
  17. Winschnitzer, S.: Application of electron microscope preparation to histological light microscopy. Z. wiss. Mikr. 65, 325–329 (1963).Google Scholar

Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • C. Orfanos
    • 1
  • I. Thiele
    • 1
  1. 1.Hautklink der Universität KölnKölnDeutschland

Personalised recommendations