Psychopharmacology

, Volume 91, Issue 3, pp 297–304 | Cite as

A simple and rapid method for assessing similarities among directly observable behavioral effects of drugs: PCP-like effects of 2-amino-5-phosphonovalerate in rats

  • W. Koek
  • J. H. Woods
  • P. Ornstein
Original Investigations

Abstract

Directly observable behavioral effects of the N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-5-phosphonovalerate (AP5) (10–1,000 mg/kg IP, 0.18–5.6 μmol/rat ICV) and of phencyclidine (PCP) (3.2–56 mg/kg IP, 0.032–3.2 mg/rat ICV), ketamine (10–100 mg/kg), amphetamine (1–18 mg/kg), apomorphine (0.1–5.6 mg/kg), chlordiazepoxide (1–100 mg/kg), and pentobarbital (3.2–56 mg/kg) were studied in rats. Pharmacologically specific results were obtained rapidly and reliably, using a cumulative dosing procedure. Cluster analysis grouped the drug treatments, on the basis of their similarities in producing different behavioral activities, into three main clusters; characteristically, stimulant drugs (amphetamine, apomorphine) produced sniffing and gnawing; PCP-like drugs (PCP, ketamine) produced locomotion, sniffing, swaying and falling; sedative drugs (pentobarbital, chlordiazepoxide) produced loss of righting. The behavioral effects of ICV administration of AP5 were more similar to the effects of PCP-like drugs than to the effects of either stimulant or sedative drugs, thus supporting the hypothesis that the behavioral effects of PCP-like drugs may result from reduced neurotransmission at excitatory synapses utilizing NMDA preferring receptors. The present procedure is simple, rapid and may provide a useful approach in the classification of behaviorally active drugs.

Key words

2-Amino-5-phosphonovalerate (AP5) Excitatory amino acid antagonists Phencyclidine Ketamine Amphetamine Apomorphine Pentobarbital Chlordiazepoxide Directly observable behaviors Cumulative dosing Cluster analysis Classification Rats 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • W. Koek
    • 1
  • J. H. Woods
    • 1
    • 2
  • P. Ornstein
    • 3
  1. 1.Department of PharmacologyUniversity of MichiganAnn ArborUSA
  2. 2.Department of PsychologyUniversity of MichiganAnn ArborUSA
  3. 3.Lilly Research LaboratoriesIndianapolisUSA

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