Nutritional effects on the kinetics of ethanol production from glucose by Zymomonas mobilis

  • Sue Cromie
  • Horst W. Doelle
Industrial Microbiology


Changes in the nutritional media significantly affected specific glucose uptake rate (Qg), glucose utilization and ethanol production rate (Qp) of Zymomonas mobilis Z 10. Varying the yeast extract concentrations (pantothenate) between 0.15% and 1.0% in a 10% glucose system at μ=0.2 h−1, Qg values between 14.9 and 35.3 and Qp values between 7.4 and 14.6 were obtained. A concentration of 0.25% yeast extract gave highest results. High NH 4 + concentrations were detrimental to ethanol production, whereas phosphate, potassium and magnesium stimulated the catabolic activity. In the case of magnesium, glucose utilization increased from 2.8 to 92.2%. Batch and continuous cultures showed that by increasing catabolic activity, ethanol productivity can be increased irrespective of glucose concentration.


Ethanol Production Specific Growth Rate Ethanol Yield Glucose Utilization Zymomonas Mobilis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Belaich JP, Senez JC (1965) Influence of aeration and of pantothenate on growth yields of Zymomonas mobilis. J Bacteriol 89:1195–1200Google Scholar
  2. Belaich JP, Senez JC, Murgier M (1968) Microcalorimetric study of glucose permeation in microbial cells. J Bacteriol 95:1750–1757Google Scholar
  3. Lee KJ, Tribe DE, Rogers PL (1979) Ethanol production by Zymomonas mobilis in continuous culture at high glucose concentrations. Biotechnol Lett 1:421–426CrossRefGoogle Scholar
  4. Reichelt JL, Doelle HW (1971) The influence of dissolved oxygen concentration of phosphofructokinase and the glucose metabolism of Escherichia coli K-12. Antonie van Leeuwenhoek J Microbiol Serol 37:497–506CrossRefGoogle Scholar
  5. Rogers PL, Lee Kj, Tribe DE (1979) Kinetics of alcohol production by Zymomonas mobilis at high sugar concentrations. Biotechnol Lett 1:165–170CrossRefGoogle Scholar
  6. Romano AH, Trifone JD, Brustolon M (1979) Distribution of the phosphoenolpyruvate: glucose phosphotransferase system in fermentative bacteria. J Bacteriol 139:93–97Google Scholar
  7. Sly LI, Doelle HW (1968) Glucose 6-phosphate dehydrogenase in cell-free extract of Zymomonas mobilis. Arch Mikrobiol 63:197–213CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Sue Cromie
    • 1
  • Horst W. Doelle
    • 1
  1. 1.Department of MicrobiologyUniversity of QueenslandSt. LuciaAustralia

Personalised recommendations