Summary
The posterior hypothalamus of anaesthetized cats was superfused through a push-pull cannula with histamine agonists and antagonists and the release of endogenous catecholamines was determined in the superfusate. Hypothalamic superfusion with histamine, 2-methylhistamine (H1-agonist), dimaprit (H2-agonist) or metiamide (H2-antagonist) enhanced the release of the catecholamines dopamine, noradrenaline and adrenaline. The releasing effects of these substances depended on the presence of calcium ions. Superfusion with 2-pyridylethylamine (H1-agonist) was virtually ineffective, while superfusion with 2-thiazolethylamine (H1-agonist) enhanced the rate of release of noradrenaline and adrenaline without influencing the release of dopamine. Superfusion with mepyramine (H1-antagonist) inhibited the release of noradrenaline and adrenaline without affecting the release of dopamine. Hypothalamic superfusion with a concentration of procaine which was equi-anaesthetic to that of mepyramine was ineffective. Ranitidine (H2-antagonist) did not alter the rates of release of the catecholamines. The releasing effect of histamine was inhibited on hypothalamic superfusion with mepyramine and ranitidine. Ranitidine also inhibited the releasing effects of dimaprit and 2-methylhistamine thus indicating that the releasing action of the latter compound was mainly due to stimulation of H2-receptors. These data suggest that blockade of H1-receptors of the posterior hypothalamus reduces the release of noradrenaline and adrenaline, while stimulation of H1-receptors seems to increase the rates of release of these two catecholamines. Stimulation of H2-receptors enhances the release of all three catecholamines. Thus, dopaminergic neurones of the hypothalamus seem to possess H2-receptors, while noradrenergic and adrenergic neurones possess H1- and H2-receptors.
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References
Andén NE, Grabowska-Andén M (1980) Influence of the H2-receptor blocking agent metiamide of the clonidine-induced changes in the brain catecholamine turnover. J Neural Transm 47: 175–180
Axelrod J, Tomchick R (1958) Enzymatic O-methylation of epinephrine and other catechols. J Biol Chem 232:702–705
Bhargava KP, Kulshresta VK, Santhakumari G, Srivastava YP (1973) Mechanism of histamine-induced antidiuretic response. Br J Pharmacol 47:700–706
Brittain RT, Jack D, Price BJ (1981) Recent developments in histamine H2-antagonists. Trend Pharmacol Sci 2:310–313
Costentin J, Boulu P, Schwartz JC (1973) Pharmacological studies on the role of histamine in thermoregulation. Agents Actions 3:177
Dietl H, Eisert A, Kraus A, Philippu A (1981) The release of endogenous catecholamines in the cat hypothalamus is affected by spinal transection and drugs which change the arterial blood pressure. J Auton Pharmacol 1:279–286
Durant GJ, Ganellin CR, Parsons ME (1975) Chemical differentiation of histamine H1- and H2-receptor agonists. J Med Chem 18:905–909
Enero MN, Langer SZ (1975) Pharmacological effects of histamine on the isolated nictitating membrane. Br J Pharmacol 53: 431P-432P
Finch L, Hicks PE (1976) The cardiovascular effects of intraventricularly administered histamine in the anaesthetized rat. Naunyn-Schmiedeberg's Arch Pharmacol 293:151–157
Flacke W, Atanackovic D, Gillis RA, Alper MH (1967) The action of histamine on the mammalian heart. J Pharmacol Exp Ther 155:271–278
Green MD, Cox B, Lomax P (1976) Sites and mechanisms of action of histamine in the central thermoregulatory pathways in the rat. Neuropharmacology 15:321–324
Lokhandwala MF (1978) Inhibition of sympathetic nervous system by histamine: studies with H1- and H2-receptor antagonists. J Pharmacol Exp Ther 206:115–122
Marco EJ, Balfagón G, Martîn J, Gómez B, Lluch S (1980) Indirect adrenergic effect of histamine in cat cerebral arteries. Naunyn-Schmiedeberg's Arch Pharmacol 312:239–243
McGrath MA, Shepherd JT (1976) Inhibition of adrenergic neurotransmission in canine vascular smooth muscle by histamine. Mediation by H2-receptors. Circ Res 39:566–573
Naranjo P, De Naranjo EB (1958) Local anaesthetic activity of some antihistamines and its relationship with the antihistamimic and anticholinergic activities. Arch Int Pharmacodyn CXIII:313–335
Philippu A, Wiedemann K (1981) Hypothalamic superfusion with histamine agonists and antagonists modifies the pressor response to hypothalamic stimulation. J Auton Pharmacol 1:111–117
Philippu A, Przuntek H, Roensberg W (1973) Superfusion of the hypothalamus with gamma-aminobutyric acid: effect on release of noradrenaline and blood pressure. Naunyn-Schmiedeberg's Arch Pharmacol 276:103–118
Philippu A, Dietl H, Sinha JN (1979) In vivo release of endogenous catecholamines in the hypothalamus. Naunyn-Schmiedeberg's Arch Pharmacol 308:137–142
Rand MJ, Story DF, Wong-Dusting HK (1982) Effects of histamine on the resting and stimulation-induced release of (3H)-noradrenaline in guinea-pig isolated atria. Br J Pharmacol 75:57–64
Sinha JN, Gupta ML, Bhargava KP (1969) Effect of histamine and antihistaminics on central vasomotor loci. Eur J Pharmacol 5:235–238
Sinha JN, Dietl H, Philippu A (1980) Effect of a fall of blood pressure on the release of catecholamines in the hypothalamus. Life Sci 26:1751–1760
Snider RS, Niemer WT (1961) A stereotaxic atlas of the cat brain. The University of Chicago Press, Chicago
Starke K, Weitzell R (1978) Is histamine involved in the sympathomimetic effect of nicotine? Naunyn-Schmiedeberg's Arch Pharmacol 304:237–248
Subramanian N, Mulder AH (1977) Modulation by histamine of the efflux of radiolabelled catecholamines from rat brain slices. Eur J Pharmacol 43:143–152
Trendelenburg U (1957) Stimulation of sympathetic centres by histamine. Circ Res 5:105–110
Tuomisto L, Eriksson L (1974) Central antidiuretic effect of histamine in the unanaesthetized goat; effects of H1 and H2 antagonists. Pharmacol (Paris) 5:Suppl 2, 101
Tuomisto J, Tuomisto L (1980) Effects of histamine antagonists on the uptake and release of catecholamines and 5-HT in brain synaptosomes. Med Biol 58:33–37
Watanabe T, Taguchi Y, Hayashi H, Tanaka J, Shiosaka S, Tohyama M, Kubota H, Terano Y, Wada H (1983) Evidence for the presence of a histaminergic neuron system in the rat brain: an immunohistochemical analysis. Neurosci Lett 39:249–254
White T (1965) Peripheral vascular effects of histamine administered into lateral ventricles of anaesthetized cats. Experientia (Basel) 21:132–133
Wilcox BJ, Seybold VS (1982) Localization of neuronal histamine in rat brain. Neurosci Lett 29:105–110
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This work was supported by the Deutsche Forschungsgemeinschaft
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Philippu, A., Bald, M., Kraus, A. et al. In vivo release by histamine agonists and antagonists of endogenous catecholamines in the cat hypothalamus. Naunyn-Schmiedeberg's Arch. Pharmacol. 326, 116–123 (1984). https://doi.org/10.1007/BF00517307
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DOI: https://doi.org/10.1007/BF00517307