Summary
Rat brain frontal cortex or striatal slices preincubated with 3H-serotonin were superfused with physiological salt solution and tritium overflow was evoked by electrical field stimulation at a frequency of 3 Hz.
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1.
GABA 1 mmol/l inhibited the evoked overflow from frontal cortex slices by about 30%. The inhibitory effect was abolished when the frequency of electrical stimulation was 10 instead of 3 Hz. Progabide and R(−)-baclofen were more potent, whereas muscimol was less potent than GABA itself in inhibiting the evoked overflow; the IC15 values of progabide, R(−)-baclofen, GABA and muscimol were 8.3, 15, 170 and >320 μmol/l, respectively. S(+)-baclofen behaved as a partial agonist with a maximum inhibitory effect by about 15%. Nipecotic acid and aminooxyacetic acid were ineffective. The same held true for bicuculline, picrotoxin and diazepam.
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2.
Bicuculline, picrotoxin, diazepam, phentolamine and the serotonin receptor antagonist metitepin did not influence the inhibitory effect of GABA. By contrast, S(+)-baclofen attenuated the effects of GABA and R(−)-baclofen.
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3.
The evoked overflow from striatal slices was inhibited by GABA and progabide (IC15 values: 480 and 13 μmol/l, respectively). Nipecotic acid was ineffective.
The results suggest that exogenous GABA inhibits serotonin release in the rat brain via GABAB receptors which may be assumed to be located presynaptically.
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This study was supported by a grant of the Deutsche Forschungsgemeinschaft
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Schlicker, E., Classen, K. & Göthert, M. GABAB receptor-mediated inhibition of serotonin release in the rat brain. Naunyn-Schmiedeberg's Arch. Pharmacol. 326, 99–105 (1984). https://doi.org/10.1007/BF00517304
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DOI: https://doi.org/10.1007/BF00517304