, Volume 72, Issue 2, pp 315–319 | Cite as

A simple method for incubation of tissue sections in immunohistochemistry

  • W. Ormanns
  • U. Pfeifer
Short Communication


The construction of a simple “incubatin chamber” is presented by which a homogeneous incubation of tissue sections with calculable amounts of immunoreagents is guaranteed. The incubation of large area sections, and parallel incubations of 10 slides are possible. As an example of application a case of alpha-1-antitrypsin deficiency in the liver is demonstrated.


Public Health Tissue Section Area Section Calculable Amount Parallel Incubation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bartonicek V, Lojda Z (1963) Incubation chambers made of plastic. Acta Histochem 15:389–391Google Scholar
  2. Graham RC, Karnowsky MJ (1966) The early stages of absorption of injected horse radish peroxidase in proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302Google Scholar
  3. Lojda Z, Gossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New YorkGoogle Scholar
  4. Lieberman J, Mittman C, Gordon HW (1972) Alpha-1-antitrypsin in the livers of patients with emphysema. Science 175:63–65Google Scholar
  5. Palmer PE, Wolfe HJ, Dayal Y, Gang DL (1978) Immunocytochemical diagnosis of alpha-1-antitrypsin deficiency. Am J Surg Pathol 2:275–281Google Scholar
  6. Sternberger L (1974) Immunocytochemistry 2nd ed. John Wiley and Sons, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • W. Ormanns
    • 1
  • U. Pfeifer
    • 1
  1. 1.LuitpoldkrankenhausPathologisches Institut der Universität WürzburgWürzburgFederal Republic of Germany

Personalised recommendations