Histochemistry

, Volume 72, Issue 2, pp 315–319 | Cite as

A simple method for incubation of tissue sections in immunohistochemistry

  • W. Ormanns
  • U. Pfeifer
Short Communication

Summary

The construction of a simple “incubatin chamber” is presented by which a homogeneous incubation of tissue sections with calculable amounts of immunoreagents is guaranteed. The incubation of large area sections, and parallel incubations of 10 slides are possible. As an example of application a case of alpha-1-antitrypsin deficiency in the liver is demonstrated.

Keywords

Public Health Tissue Section Area Section Calculable Amount Parallel Incubation 

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References

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  3. Lojda Z, Gossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New YorkGoogle Scholar
  4. Lieberman J, Mittman C, Gordon HW (1972) Alpha-1-antitrypsin in the livers of patients with emphysema. Science 175:63–65Google Scholar
  5. Palmer PE, Wolfe HJ, Dayal Y, Gang DL (1978) Immunocytochemical diagnosis of alpha-1-antitrypsin deficiency. Am J Surg Pathol 2:275–281Google Scholar
  6. Sternberger L (1974) Immunocytochemistry 2nd ed. John Wiley and Sons, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • W. Ormanns
    • 1
  • U. Pfeifer
    • 1
  1. 1.LuitpoldkrankenhausPathologisches Institut der Universität WürzburgWürzburgFederal Republic of Germany

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