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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 306, Issue 1, pp 37–44 | Cite as

Substance P in the vagus nerve

Immunochemical and immunohistochemical evidence for axoplasmic transport
  • R. Gamse
  • F. Lembeck
  • A. C. Cuello
Article

Summary

  1. 1.

    The presence of immunoreactive substance P (I-SP) in the vagus nerve of 5 species was demonstrated by radioimmunoassay. Different amounts of SP per unit weight were found: Guinea pig > cat > rabbit, rat and cattle.

     
  2. 2.

    Infranodose ligations of the vagus nerve of cats and rabbits caused an accumulation of I-SP proximal but not distal to the ligation. The results obtained by radioimmunoassay and immunohistochemistry indicate a somatofugal axoplasmic transport of SP.

     
  3. 3.

    Double ligation experiments revealed that about 37% of I-SP of the cat vagus nerve are mobile. The transport rate of this mobile I-SP was found to be 170 mm per day.

     
  4. 4.

    Locally applied colchicine resulted in a similar accumulation of I-SP as after ligations. This is evidence favouring the involvement of microtubuli in the axoplasmic transport of SP.

     
  5. 5.

    Immunohistochemical data show that SP-fibers account for about 10% of the axons in the cat vagus nerve. Most of these SP-fibers seem to be unmyelinated.

     
  6. 6.

    Supranodose extracranial ligations of the cat vagus nerve led to an accumulation of I-SP on both sides of the ligature. Part of the SP-fibers are, therefore, afferent and their cell bodies are located in the nodose ganglion. The presence of efferent SP-fibers cannot be excluded.

     

Key words

Substance P Vagus nerve Axoplasmic transport 

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • R. Gamse
    • 1
  • F. Lembeck
    • 1
  • A. C. Cuello
    • 2
  1. 1.Institut für Experimentelle und Klinische PharmakologieGrazAustria
  2. 2.MRC Neurochemical Pharmacology UnitDepartment of PharmacologyCambridgeEngland

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