Summary
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1.
The effect of neuropeptide Y (NPY) on fractional tritium-noradrenaline (3H-NA) release and contractile activity was studied in the isolated portal vein of SHR and WKY rats.
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2.
NPY (5×10−7M) enhanced the force of the spontaneous contractile activity by about 40%.
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3.
The fractional 3H-release elicited by transmural nerve stimulation (TNS), which mainly reflects 3H-NA, was reduced by about 40% after preincubation with 5×10−7 M NPY in portal veins from both SHR and WKY rats. The inhibitory effect of NPY on TNS-evoked 3H-release was more slowly reverved by washout than the facilitatory action on spontaneous contractile force.
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4.
The contractile response to field stimulation was not reduced by NPY, but rather tended to be increased.
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5.
It is concluded that NPY exerts a dual action in the SHR and WKY portal vein, thus enhancing the smooth muscle contractions and inhibiting sympathetic neurotransmission. The inhibitory effect of NPY on TNS-evoked NA efflux, which is present in both SHR and WKY rats is most likely due to a presynaptic site of action.
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Dahlöf, C., Dahlöf, P., Tatemoto, K. et al. Neuropeptide Y (NPY) reduces field stimulation-evoked release of noradrenaline and enhances force of contraction in the rat portal vein. Naunyn-Schmiedeberg's Arch. Pharmacol. 328, 327–330 (1985). https://doi.org/10.1007/BF00515562
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DOI: https://doi.org/10.1007/BF00515562