Effect of short-term strenuous exercise on erythrocyte 2,3-diphosphoglycerate in untrained men: a time-course study

  • Omokere E. Odje
  • James M. Ramsey
Original Article

Abstract

The literature on the response of erythrocyte 2,3-diphosphoglycerate (2,3-DPG) following exercise is replete with inconsistencies, and recent studies have shown that the time of blood sampling during and following exercise, as well as the duration of exercise, are important in evaluating the response of 2,3-DPG. Experiments were designed to measure the response of 2,3-DPG following short-term strenuous exercise in two groups of untrained men. Twelve men, 19–22 years old (study 1), exercised on a bicycle ergometer at 122.5 W for 10 min and red blood cell (RBC) 2,3-DPG was measured at 0 and 50 min following exercise. The level of 2,3-DPG (μmol · ml−1 RBC) increased after exercise (P < 0.05), but this increase was not significant when 2,3-DPG was expressed as mol · mol−1 hemoglobin (Hb). However, following 50min of rest, 2,3-DPG (mol · mol−1 Hb) decreased significantly. In a second group (study 2), nine other men, aged 18–19 years, exercised at the same workload for 15 min and 2,3-DPG was measured at 0, 30, 60, 180, and 330 min respectively after exercise, and no significant mean changes in the level of the phosphate were observed. Findings from these studies suggest that 2,3-DPG does not provide a compensatory adjustment to facilitate oxygen delivery in the hypoxia of short-term strenuous exercise in untrained males immediately following exercise and when recovery intervals of up to 330min are also examined. It is suggested that 2,3-DPG be reported as mol · mol−1 Hb, since the phosphate exists on Hb in an equimolar ratio in normal physiological states.

Key words

Acute exercise 2,3-Diphosphoglycerate 2,3-bisphosphoglycerate Erythrocytes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alvarez AI, Prieto JG, Albi J, Sanchez J (1992) Erythrocyte metabolism in exercise. A comparative study in anaemized rats. J Sports Med Phys Fitness 32:314–201Google Scholar
  2. Benesch R, Benesch RE (1967) The effect of organic phosphates from human erythrocyte on the allosteric properties of hemoglobin. Biochem Biophys Res Commun 26:162–167Google Scholar
  3. Bonner HW, Tate CA, Buffington CK (1975) Changes in erythrocyte 2,3-diphosphoglycerate in women following short term maximal exercise. Eur J Appl Physiol 34:227–232Google Scholar
  4. Brauman KM, Boning D, Trost F (1982) Bohr effect and slope of the oxygen curve after physical training. J Appl Pysiol 52:1534–1539Google Scholar
  5. Brodthagen UA, Hansen J, Knudsen JB, Jordal R, Kristensen O, Paulev PE (1985) Red cell 2,3-DPG, ATP, and mean cell volume in highly trained athletes. Eur J Appl Physiol 53:334–338Google Scholar
  6. Brown SP, Keith WB (1993) The effects of acute exercise on levels of erythrocyte 2, 3-bisphosphoglycerate: a brief review. J Sports Sci 11:479–484Google Scholar
  7. Donovan CA, Pagliassotti MJ (1990) Enhanced efficiency of lactate removal after endurance training. J Appl Physiol 68:1053–1058Google Scholar
  8. Katz A, Sharp RL, King DS, Costill DL, Fink WJ (1984) Effect of high intensity interval training on 2,3-diphosphoglcerate at rest and after maximal exercise. Eur J Appl Physiol 52:331–335Google Scholar
  9. Lijnen P, Hespel P, Van Oppens S, Fiocchi R, Goossens W, Vanden Eynde E, Amery A (1986) Erythrocyte 2,3-diphosphoglycerate and serum concentrations in trained and sedentary men. Med Sci Sports Exerc 18:174–179Google Scholar
  10. Lijnen P, Hespel R, Fargard R, Lysens R, Vanden Eynde E, Goris M, Amery A (1988) Erythrocyte 2,3-diphosphoglycerate concentration before and after a marathon in men. Eur J Appl Physiol 57:452–455Google Scholar
  11. Mairbaurl H, Humpeler E, Schwaberger G, Pessenhofer H (1983) Training-dependent changes of red cell density and erythrocytic oxygen transport. J Appl Physiol 55:1403–1407Google Scholar
  12. Meen HD, Hotter PH, Refsum HE (1981) Changes in 2,3-diphosphoglycerate after exercise. Eur J Appl Physiol 46:177–184Google Scholar
  13. Quatrini U, Licciardi A, Morici G (1993) Oxygen-hemoglobin dissociation curve in hypoxic rats of first or second generation. Clin Exp Pharmacol Physiol 20:269–274Google Scholar
  14. Ramsey JM, Pipoly SW Jr (1979) Response of erythrocyte 2,3diphosphoglycerate to strenuous exercise. Eur J Appl Physiol 40:227–233Google Scholar
  15. Reinhart WH, Staubli M, Straub PW (1983) Impaired red cell filterability with ellimination of old red blood cells during a 100 km race. Eur J Appl Physiol 54:827–830Google Scholar
  16. Remes K, Harkonen M, Vuopio P, Peltokallio P (1975) The decrease in red cell 2, 3-diphosphoglycerate concentration in long distance running. J Sports Med 15:113–116Google Scholar
  17. Remes K, Vuopio P, Harkonen M (1979) Effect of long-term training and acute physical exercise on red cell 2, 3-diphosphoglycerate. Eur J Appl Physiol 42:199–207Google Scholar
  18. Rose ZB (1970) Enzyme controlling 2,3-diphosphoglycerate in human erythrocyte Fed Proc 29:1105–1111Google Scholar
  19. Rose ZB, Liebowitz J (1970) Direct determination of 2,3-diphosphoglycerate. Ann Biochem 35:177–180Google Scholar
  20. Seaman C, Wyss S, Piomelli S (1980) The decline in energetic metabolism with aging of erythrocyte and its relationship to cell death. Am J Hematol 8:31–42Google Scholar
  21. Sokal RR, Rohlf FJ (1981) Biometry. The principles and practice of statistics in biological research. Freeman, San FranciscoGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Omokere E. Odje
    • 1
  • James M. Ramsey
    • 2
  1. 1.Department of BiologyUniversity of DaytonDaytonUSA
  2. 2.Biology DepartmentCentral State UniversityWilberforceUSA

Personalised recommendations