Skip to main content
SpringerLink
Log in

A streamlined technique for on-grid electron immunocytochemistry

  • Short Communication
  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

The unlabelled antibody enzyme technique for on-grid immunostaining demands exacting conditions of cleanliness. These can be met with minimal trouble by two technical modifications.

Large supplies of all the solutions are millipore-filtered in advance and are then stored frozen in sealed plastic bags and allowed to thaw when required.

A disposable, contamination-free surface with depressions to hold drops of staining solution is prepared from a strip of parafilm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Sternberger, L.A.: Immunocytochemistry. Englewood Cliffs, N.J., U.S.A. Prentice-Hall, Inc. 1974

    Google Scholar 

Download references

Author information

Author notes

  1. Lauri J. Pelliniemi

    Present address: Laboratory of Electron Microscopy, University of Turku, SF-20520, Turku 52, Finland

Authors and Affiliations

  1. Department of Histochemistry, Royal Postgraduate Medical School, W12 OHS, London

    Lauri J. Pelliniemi & Susan Van Noorden

Authors
  1. Lauri J. Pelliniemi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susan Van Noorden
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pelliniemi, L.J., Van Noorden, S. A streamlined technique for on-grid electron immunocytochemistry. Histochemistry 53, 271–272 (1977). https://doi.org/10.1007/BF00511083

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00511083

Keywords

Search

Navigation