Skip to main content

Alterations in leukocyte aryl hydrocarbon hydroxylase activity associated with treatment and age in psoriasis patients and healthy individuals

Archives of Dermatological Research volume 276, pages 105–110 (1984)Cite this article

Summary

We have examined the relationship between topical psoriasis treatment and the activity of the mixed function oxidase, aryl hydrocarbon hydroxylase (AHH), in peripheral blood monocytes and lymphocytes from 20 patients with psoriasis. These data were compared to monocyte and lymphocyte AHH activity measured in 20 normal subjects. AHH activity was determined in cells induced with benzanthracene and in uninduced control cells. Monocyte and lymphocyte AHH activity in six untreated psoriasis patients was similar to that in the healthy controls. AHH activity in either uninduced or induced monocytes showed an increase with age in both the healthy and the untreated psoriatic subjects. Lymphocytes from the healthy subjects showed an age-related decline in enzyme activity. Fourteen patients with psoriasis receiving topical tar and/or topical corticosteroid therapy had significantly higher (P<0.05) levels of basal and induced monocyte and lymphocyte AHH activity than the healthy controls. AHH activity is age-related and appears to be controlled differently in monocytes and in lymphocytes. AHH activity in circulating monocytes and lymphocytes may be stimulated by topical tar and/or steroid therapy of psoriasis.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. Bast Jr RC, Okuda T, Plotkin E, Tarone R, Rapp HJ, Gelboin HV (1976) Development of an assay for aryl hydrocarbon (benzo[a]pyrene) hydroxylase in human peripheral blood monocytes. Cancer Res 36:1967–1974

    Google Scholar 

  2. Bast Jr RC, Bast BS, Gelboin HV (1980) Metabolism of polycyclic aromatic hydrocarbons by lymphoreticular cells. In: Gram T, Vessel E, Garattini S (eds) Extrahepatic metabolism of drugs and other foreign compounds, vol 5. Monographs in pharmacology and physiology. Spectrum Publications, New York, pp 277–294

    Google Scholar 

  3. Bickers DR, Kappas A (1978) Human skin aryl hydrocarbon hydroxylase induction by coal tar. J Clin Invest 62:1061–1068

    Google Scholar 

  4. Bickers DR, Kappas A (1980) The skin as a site of chemical metabolism. In: Gram TE, Vessel E, Garattini S (eds) Extrahepatic metabolism of drugs and other foreign compounds. Monographs in pharmacology and physiology. Spectrum Publications, New York, pp 295–318

    Google Scholar 

  5. Bickers DR, Wroblewski D, Tapu-Dutta-Choudhury, Mukhtar H (1982) Induction of neonatal rat skin and liver aryl hydrocarbon hydroxylase by coal tar and its constituents. J Invest Dermatol 78:227–229

    Google Scholar 

  6. Bickers D, Mukhtar H, Dutta-Choudhury T, Marcelo C, Voorhees J (1982) Epidermal drug metabolism in human subjects: Comparative activity in normal individuals and patients with psoriasis. Clin Res 30:260a

    Google Scholar 

  7. Briggs MH, Briggs M (1973) Induction by topical corticosteroids of skin enzymes metabolizing carcinogenic hydrocarbons. Brit J Dermatol 88:75–81

    Google Scholar 

  8. Chapman PH, Rawlins MD, Shuster S (1979) Activity of aryl hydrocarbon hydroxylase in psoriatic skin. Lancet I:297–298

    Google Scholar 

  9. Fujino T, Park SS, West D, Gelboin HV (1982) Phenotyping of cytochromes P-450 in human tissues with monoclonal antibodies. Proc Natl Acad Sci USA 79:3682–3686

    Google Scholar 

  10. Gelboin HV (1980) Benzo(a)pyrene metabolism, activation, and carcinogenesis: Role and regulation of mixed-function oxidases and related enzymes. Physiol Rev 60:1107–1166

    Google Scholar 

  11. Karki NT, Karvonen J, Pelkonen O (1982) Aryl hydrocarbon hydroxylase activity in cultured lymphocytes of psoriatic patients. Arch Dermatol Res 273:103–109

    Google Scholar 

  12. Kellermann G, Shaw CR, Luyten-Kellerman M (1973) Aryl hydrocarbon hydroxylase inducibility and bronchogenic carcinoma. N Engl J Med 289:934–937

    Google Scholar 

  13. Okano P, Miller HN, Robinson RC, Gelboin HV (1979) Comparison of benzo[a]pyrene and(-)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene metabolism in human blood monocytes and lymphocytes. Cancer Res 39:3184–3193

    Google Scholar 

  14. Okuda T, Vesell ES, Tarone R, Bast C, Gelboin HV (1977) Interindividual and intraindividual variation in aryl hydrocarbon hydroxylase in monocytes from monozygotic and dizygotic twins. Cancer Res 37:3904–3911

    Google Scholar 

  15. Paigen B, Gurtoo HL, Minowada J (1977) Questionable relation of aryl hydrocarbon hydroxylase to lung cancer risk. N Engl J Med 297:346–350

    Google Scholar 

  16. Paigen B, Gurtoo H, Minowada J, Ward E, Houten L, Paigen K, Reilly A, Vincent R (1978) Genetics of aryl hydroxylase in human population and its relationship to lung cancer. In: Gelboin HV, Tso POP (eds) Polycyclic hydrocarbons and cancer, vol 2. Academic Press, New York, pp 391–406

    Google Scholar 

  17. Rahimtula AD, Zachariah PK, O'Brien PJ (1979) Differential effects of antioxidants, steroids and other compounds on benzo(a)pyrene 3-hydroxylase activity in various tissues of rat. Br J Cancer 40:105–112

    Google Scholar 

  18. Rawlins M, Shuster S (1982) Aryl hydrocarbon hydroxylase in psoriasis. Lancet II:271

    Google Scholar 

  19. Ruhmann-Wennhold A, Nelson DH (1978) An effect of corticosteroids and 100% oxygen on aryl hydrocarbon hydroxylase, cytochrome-c reductase and free radical formation by rat lung microsomes. Metabolism 27:1013–1022

    Google Scholar 

  20. Ruzicka T, Vizethum W, Jacobs A, Goetz G (1980) Aryl hydrocarbon hydroxylase activity in lymphocytes of patients with psoriasis. Lancet I:1142–1143

    Google Scholar 

  21. Slaga TJ, Viaje A, Bracken W (1977) The effects of antiinflammatory agents on skin tumor initiation and aryl hydrocarbon hydroxylase. Res Comm Chem Path Pharm 16:337–350

    Google Scholar 

  22. Somogyi A, Kovacs K, Solymoss B, Kuntzman R, Conney AH (1971) Suppression of 7,12-dimethylbenz(alpha)anthraceneproduced adrenal necrosis by steroids capable of inducing aryl hydrocarbon hydroxylase. Life Sci 10 (part II):1261–1271

    Google Scholar 

  23. Stern RS, Zierler S, Parrish JA (1980) Skin carcinoma in patients treated with topical tar and artificial ultraviolet radiation. Lancet I:732–735

    Google Scholar 

  24. Whitlock JP, Miller H, Gelboin HV (1974) Induction of aryl hydrocarbon (benzo[a]pyrene] hydroxylase and tyrosine aminotransferase in hepatoma cells in cultures. J Cell Biol 63:136–145

    Google Scholar 

  25. Weibl FJ, Gelboin HV (1977) Cutaneous carcinogenesis: Metabolic interaction of chemical carcinogens with skin. In: Lee DHK (ed) Handbook of physiology. Environmental physiology: Reactions to environmental agents. William and Wikins, Baltimore, pp 337–348

    Google Scholar 

Download references

Author information

Affiliations

  1. Laboratory of Molecular Carcinogenesis National Cancer Institute, National Institutes of Health, 20205, Bethesda, MD, USA

    K. H. Kraemer, R. C. Robinson, M. Protic-Sabljic & H. V. Gelboin

  2. Biometry Branch, National Cancer Institute, National Institutes of Health, 20205, Bethesda, MD, USA

    R. E. Tarone

  3. Center for Marine Research, Rudjer Boskovic Institute, Zagreb, Yugoslavia

    M. Protic-Sabljic

Authors
  1. K. H. Kraemer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. C. Robinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. E. Tarone
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Protic-Sabljic
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. V. Gelboin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kraemer, K.H., Robinson, R.C., Tarone, R.E. et al. Alterations in leukocyte aryl hydrocarbon hydroxylase activity associated with treatment and age in psoriasis patients and healthy individuals. Arch Dermatol Res 276, 105–110 (1984). https://doi.org/10.1007/BF00511066

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00511066

Key words

  • Aryl hydrocarbon hydroxylase
  • Psoriasis
  • Leukocytes
  • Age
  • Topical therapy