Zusammenfassung
Das Molluscum-contagiosum-Virus wurde in Glutaraldehyd und OsO4 fixiert und in Vestopal W eingebettet. Die genaure nur mit dem Elektronenmikroskop erkennbare Struktur des Virus wurde durch zweifache Färbung und zwar mit Uranylacetat und Bleihydroxyd dargestellt.
In ultradünnen Schnitten wurde das Molluscum-contagiosum-Virus bis auf die Außenmembran durch Pronase und Nagarse verdaut. Durch Verdauung mit Pronase war die dreischichtige Struktur der äußeren Membran deutlich zu sehen.
Summary
Molluscum cantagiosum virus was fixed in glutaraldehyde and OsO4 embedded in Vestopal W. More detailed sultrastructure of virus was shownby double staining with uranyl acetate and lead hydroxide.
Molluscum contagiosum virus in ultrathin section was digested comletely except for remaining of outer membrane with Pronase and Nagarse. Triple layer structure of outer membrane was clearly seen by digestion with Pronase.
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Hasegawa, T., Fujiwara, E., Ametani, T. et al. Further electron microscopic observation of molluscum contagiosum virus. Arch. klin. exp. Derm. 235, 319–328 (1969). https://doi.org/10.1007/BF00510970
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DOI: https://doi.org/10.1007/BF00510970