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Lanthanum staining of the epidermal intercellular spaces

An electron microscopic study

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Zusammenfassung

Eine sich mit Lanthan färbende Substanz, wie sie kürzlich von Wolff u. Schreiner in den intercellulären Räumen der Epidermis beschrieben worden ist, kann auch in Schnitten nachgewiesen werden, die mit Lanthan-Glutaraldehyd fixiert wurden. Bei Fixierung in Glutaraldehyd allein entspricht die lanthan-positive Substanz in den intercellulären Räumen nicht vollständig der desmosomestruktur. Der mögliche chemische Unterschied zwischen der Substanz im intercellulären Raum und dem im desmosome wird in der vorliegenden Arbeit erörtert.

Summary

Lanthanum-staining substance, which has been recently reported by Wolff and Schreiner in the epidermal intercellular spaces, can also be demonstrated in lanthanum-glutaraldehyde fixed sections. With fixation in glutaraldehyde alone, the lanthanum-staining substance in the intercellular spaces is not fully continuous with the desmosome structure. The possible chemical difference between the substance in the intercellular space and that in the desmosome is discussed in this paper.

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References

  1. Braun-Falco, O., u. W. Vogell: Elektronenmikroskopische Untersuchungen zur Dynamik der Acantholyse bei Pemphigus vulgaris. I. Die klinisch normal aussehende Haut in der Umgebung von Blasen mit positivem Nikolski-Phänomenen. Arch. klin. exp. Derm. 223, 328–346 (1965).

    Google Scholar 

  2. — u. G. Petry: Zur Feinstruktur der Epidermis bei chronischem nummulären Ekzem. II. Mitteilung: Stratum spinosum. Arch. klin. exp. Derm. 224, 63–80 (1966)

    Google Scholar 

  3. Cancilla, P. A.: Demonstration of the Langenhans granule by lanthanum. J. Cell Biol. 38, 248–252 (1968).

    Google Scholar 

  4. Doggenweiler, C. F., and S. Frenk: Staining properties of lanthanum on cell membranes. Proc. nat. Acad. Sci. (Wash). 53, 425–430 (1965).

    Google Scholar 

  5. Kelly, D. E.: Fine structure of desmosomes, hemidesmosomes, and an adepidermal globular layer in developing newt epidermis. J. Cell Biol. 28, 51–72 (1966).

    Google Scholar 

  6. Komura, J., and S. Ofuji: Effects of electron stains and desmosome; an electron microscope study. J. invest. Derm. 48, 304–308 (1967).

    Google Scholar 

  7. ——: Phosphotungstic acid stain and epidermal cell surface. An electron microscopic study. J. invest. Derm. 49, 391–395 (1967).

    Google Scholar 

  8. Lesseps, R. J.: The removal by phospholipase C of a layer of lanthanumstaining material external to the cell membrane in embryonic chick cells. J. Cell Biol. 34, 173–183 (1967).

    Google Scholar 

  9. Mercer, E. H., R. A. Jahn, and H. I. Maibach: Surface coats containing polysaccharides on human epidermal cells. J. invest. Derm. 51, 204–214 (1968).

    Google Scholar 

  10. Mirolli, M. A., and J. W. Crayton: Lanthanum staining of the extracellular space of molluscan ganglia. J. Cell. Biol. 39, 92a (1968).

    Google Scholar 

  11. Odland, G. F.: The fine structure of the interrelationship of cells in the human epidermis. J. biophys. biochem. Cytol. 4, 529–538 (1958).

    Google Scholar 

  12. Ofuji, S., and K. Tabata: A study on ultramicroscopic features of acute eczema. Acta derm. (Kyoto) 56, 225–227 (1961).

    Google Scholar 

  13. Overton, J.: Localized lanthanum staining of the intestinal brush border. J. Cell Biol. 38, 447–452 (1968).

    Google Scholar 

  14. Revel, J. P., and M. J. Karnovsky: Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33, C7-C12 (1967).

    Google Scholar 

  15. Schatzki, P. F.: Bile canaliculus and space of disse: Ultramicroscopic relationships as delineated by lanthanum deposits. J. Histochem. Cytochem. 16, 504 (1968).

    Google Scholar 

  16. Wolff, K., and E. Schreiner: An electron microscopic study on the extraneous coat of keratinocytes and the intercellular space of the epidermis. J. invest. Derm. 51, 418–430 (1968).

    Google Scholar 

  17. Zobel, C. R., and M. Beer: The use of heavy metal salts as electron stains. Int. Rev. Cytol. 18, 363–400 (1965).

    Google Scholar 

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Komura, J., Shimada, Y. & Ofuji, S. Lanthanum staining of the epidermal intercellular spaces. Arch. klin. exp. Derm. 235, 221–227 (1969). https://doi.org/10.1007/BF00510959

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  • DOI: https://doi.org/10.1007/BF00510959

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