Summary
Unilateral adrenal denervation caused a gradual decrease of adenylate cyclase activity in rat adrenal medulla. The extent of the increase in adrenal medullary 3′,5′-cyclic adenosine monophosphate (cAMP) content elicited by injections of carbamylcholine declined gradually following adrenal denervation. Three or nine days after denervation carbamylcholine caused rise of cAMP and a delayed increase of tyrosine-3-mono-oxygenase (TH) activity of similar magnitude in intact and denervated adrenal medullae. However, after an interval of 15 days or longer following denervation the increase in TH activity elicited by carbamylcholine was greatly reduced. These results support previous proposals that cAMP is involved as a second messenger in the trans-synaptic induction of TH.
References
Costa, E., Guidotti, A., Hanbauer, I.: Do cyclic nucleotides promote the transsynaptic induction of tyrosine hydroxylase? Life Sci. 14, 1169–1188 (1974)
Costa, E., Guidotti, A., Hanbauer, I.: Cyclic nucleotides and trophism of secretory cells: study of adrenal medulla, In: Cyclic nucleotides in disease (Ed. Weiss). Philadelphia Press 1975
Douglas, W. W., Rubin, R. P.: Mechanism of nicotinic action at the adrenal medulla: Calcium as a link in stimulus-secretion coupling. Nature (Lond.) 192, 1087–1089 (1961)
Goridis, C., Neff, N. H.: Monoamine oxidase: An approximation of turnover rates. J. Neurochem. 18, 1673–1682 (1971)
Guidotti, A., Costa, E.: Involvement of adenosine 3′,5′-monophosphate in the activation of tyrosine hydroxylase elicited by drugs. Science 179, 902–904 (1973)
Guidotti, A., Costa, E.: A role of a nicotinic receptor in the regulation of the adenylate cyclase of adrenal medulla. J. Pharmacol. exp. Ther. 189, 665–675 (1974)
Guidotti, A., Hanbauer, I., Costa, E.: Role of cyclic nucleotides in the induction of tyrosine hydroxylase: A study using the adrenal medulla and the sympathetic ganglion. Advances in Cyclic Nucleotide Res., Vol. 5 (Ed. Drummond, Greengard and Robinson). New York: Raven Press (in press, 1975a)
Guidotti, A., Kurosawa, A., Chuang, D. M., Costa, E.: Protein kinase activation as an early event in the trans-synaptic induction of tyrosine-3-mono-oxygenase in adrenal medulla. Proc. nat. Acad. Sci. (Wash.) (in press, 1975b)
Guidotti, A., Mao, C. C., Costa, E.: Trans-synaptic regulation of tyrosine hydroxylase in adrenal medulla: Possible role of cyclic nucleotides. Frontiers in Catecholamine research (Ed. Usdin and Snyder) pp. 231–236. Oxford-London: Pergamon Press 1973
Mueller, R. A., Thoenen, H., Axelrod, J.: Increase of tyrosine hydroxylase activity after reserpine administration. J. Pharmacol. exp. Ther. 169, 74–79 (1969)
Patrick, R. L., Kirshner, N.: Effect of the stimulation on the levels of tyrosine hydroxylase, dopamine-β-hydroxylase and catecholamines in intact and denervated rat adrenal glands. Molec. Pharmacol. 7, 87–96 (1971)
Schrier, B. K., Shuster, L.: A simplified radiochemical assay for choline acetyltransferase. J. Neurochem. 14, 977–985 (1967)
Shiman, R., Akino, M., Kaufman, S.: Solubilization and partial purification of tyrosine hydroxylase from bovine adrenal medulla. J. biol. Chem. 246, 1330–1340 (1971)
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Hanbauer, I., Guidotti, A. Further evidence for a cAMP dependent regulation of tyrosine-3-monoxygenase induction in adrenal medulla. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, 213–217 (1975). https://doi.org/10.1007/BF00510452
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DOI: https://doi.org/10.1007/BF00510452