Archives of Dermatological Research

, Volume 274, Issue 1–2, pp 169–177 | Cite as

Isolation and characterization of horny cell membrane

  • Tesuya Hirotani
  • Motomu Manabe
  • Hideoki Ogawa
  • Kimie Murayama
  • Tomoko Sugawara
Original Contributions


The cornified cell membrane and thickened marginal band were obtained from human stratum corneum by a new, faster procedure involving extraction, tripsinization, and density gradient centrifugation. Observations revealed a fine membranous structure with a thickened inner surface of approximately 150 Å (marginal band). Each of the membranous samples contained a level of half-cystines markedly higher in amount than that of earlier findings by Matoltsy and Matotsy [5]. The samples also contained high amounts of glutamic acid and lysine residues many of which constitute the ε-(γ-glutamyl) lysine cross-linkages of the cell membrane. This study presents the simple isolation procedure and its results highlight the high content of half-cystines in the basic membrane fraction. In addition, the study reveals the importance of S-S cross-linkages during the formation of the cell membrane.

Key words

Horny cell membrane 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Tesuya Hirotani
    • 1
  • Motomu Manabe
    • 1
  • Hideoki Ogawa
    • 1
  • Kimie Murayama
    • 2
  • Tomoko Sugawara
    • 2
  1. 1.Department of Dermatology, School of MedicineJuntendo UniversityTokyoJapan
  2. 2.Central Laboratory of Medical Science, School of MedicineJuntendo UniversityTokyoJapan

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