Archives of Dermatological Research

, Volume 277, Issue 6, pp 444–447 | Cite as

Epidermal synthesis and expression of HLA-DR on keratinocytes in lupus erythematosus

  • P. Kaudewitz
  • T. Ruzicka
  • M. Meurer
  • P. Rieber
  • O. Braun-Falco
Original Contributions

Summary

It has been suggested that aberrant expression of HLA-DR plays an important role in the induction of an autoimmune reaction. In lupus erythematosus (LE), skin represents a major target for autoimmune attack. We therefore tested lesional and nonlesional skin from 11 patients with different clinical subtypes of LE for the presence of HLA-DR molecules on keratinocytes using a monoclonal antibody against a non-polymorphic HLA-DR determinant. In all lesions tested, HLA-DR-positive keratinocytes were present, whereas in nonlesional skin, these cells remained HLA-DR negative. To exclude the possibility of passive absorption of DR molecules, the de novo synthesis of HLA-DR was demonstrated by the presence of cytoplasmic HLA-DR γ chains in keratinocytes. If aberrant HLA-DR expression is a primary event, it could facilitate the recognition of autoantigens on keratinocytes by immunocompetent cells. Alternatively, the synthesis of DR molecules could be induced as a secondary event by mediators derived from the inflammatory infiltrate.

Key words

HLA-DR expression Keratinocytes Lupus erythematosus 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bottazzo GF, Pujol-Borrell R, Hanafusa T, Feldmann M (1983) Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity. Lancet 2:1115–1118Google Scholar
  2. 2.
    Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, McDonald S, Pulford KAF, Stein H, Mason DY (1984) Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229Google Scholar
  3. 3.
    Hanafusa T, Pujol-Borrell R, Chiovato L, Russel RCG, Donaich D, Bottazzo GF (1983) Aberrant expression of HLA-DR antigen on thyrocytes in Graves' disease: relevance for autoimmunity. Lancet 2:1111–1115Google Scholar
  4. 4.
    Lambert IA (1984) Expression of HLA-DR (Ia-like) antigen on epidermal keratinocytes in human dermatoses. Clin Exp Immunol 57:57–100Google Scholar
  5. 5.
    Lambert IA, Janossy G, Suitters AJ, Borill M, Palmer S, Gordon-Smith E, Prentice HG, Thomas JA (1982) Immunological analysis of the skin in graft versus host disease. Clin Exp Immunol 50:123–131Google Scholar
  6. 6.
    Mason DW, Dallmann N, Barclay N (1981) Graft versus host disease induces the expression of Ia antigen in rat epidermal cells and gut epithelium. Nature 293:150–151Google Scholar
  7. 7.
    Messenger AG, Blehen SS, Slater DN, Rooney N (1984) Expression of HLA-DR in hair follicles in alopecia areata. Lancet 2:287–288Google Scholar
  8. 8.
    Quaranta V, Madjic O, Stingl G, Liska K, Hoenigsmann H, Knapp W (1984) A human Ia cytoplasmic determinant located on multiple forms of invariant chain (γ, γ2, γ3). J Immunol 132:1900–1905Google Scholar
  9. 9.
    Rieber P, Lohmeyer J, Schendel DJ, Riethmüller G (1981) Human T cell differentiation antigens characterizing a cytotoxic/suppressor T cell subset. Hybridoma 1:59–69Google Scholar
  10. 10.
    Stingl G, Tamaki K, Katz S (1980) Origin and function of epidermal Langerhans cells. Immunol Rev 53:149–74Google Scholar
  11. 11.
    Volc-Platzer BV, Majdic O, Knapp W, Wolff K,Hinterberger W, Lechner K, Stingl G (1984) Evidence of HLA-DR biosynthesis by human keratinocytes in disease. J Exp Med 159:1784–1789Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • P. Kaudewitz
    • 1
  • T. Ruzicka
    • 1
  • M. Meurer
    • 1
  • P. Rieber
    • 2
  • O. Braun-Falco
    • 1
  1. 1.Dermatologische Klinik und Poliklinik der Ludwig-MaximiliansUniversität MünchenMünchenFederal Republic of Germany
  2. 2.Institut für Immunologie der Ludwig-MaximiliansUniversität MünchenMünchenFederal Republic of Germany

Personalised recommendations