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Archives of Dermatological Research

, Volume 275, Issue 3, pp 181–189 | Cite as

Immunocompetent cells in psoriasis

In situ immunophenotyping by monoclonal antibodies
  • J. D. Bos
  • H. J. Hulsebosch
  • S. R. Krieg
  • P. M. Bakker
  • R. H. Cormane
Original Contributions

Summary

Immunocompetent cells in exacerbating untreated psoriasis vulgaris skin lesions were immunophenotypically studied by the application of a selection of monoclonal antibodies in a two-stage immunoperoxidase technique. Epidermal changes include: focal accumulation of immunoglobulins in the stratum corneum, as demonstrated by a mixture of monoclonal anti-kappa and anti-lambda antibodies; focal accumulation of OKM-1 positive but Mo-2 negative cells high in the epidermis, reflecting granulocytes in Munro's abcesses; a marked decrease in epidermal Langerhans cells with focal abnormal clumping and smaller dendrites, as demonstrated by monoclonal anti-HLA-DR and anti-T6 (OKT-6) antibodies; and, sporadic exocytosis of mainly T1 (Leu-1), T8 (Leu-2a) positive suppressor/cytotoxic T lymphocytes.

The dermal infiltrates were found to consist mainly of partically activated T1 (Leu-1), T4 (Leu-3a) positive T-helper/inducer cells with a smaller compartment of T1 (Leu-1), T8 (Leu-2a) positive suppressor/cytotoxic lymphocytes. These cells were found in close apposition to T6 (OKT-6), HLA-DR positive Langerhans cells and further accompanied by a minor compartment of OKM-1, Mo-2 positive monocytes. No B-cells or plasma cells could be demonstrated in the dermis. Natural killer cells were observed only incidentally.

These results fit best with the hypothesis that psoriasis is a chronic inflammatory condition as a result of persistant stimulation of T cells by immunogen(s) of epidermal origin.

Key words

Psoriasis Immunocompetent cells T-cell subsets Monoclonal antibodies 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • J. D. Bos
    • 1
  • H. J. Hulsebosch
    • 1
  • S. R. Krieg
    • 2
  • P. M. Bakker
    • 2
  • R. H. Cormane
    • 1
  1. 1.Department of Dermatology Academic Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Department of Pathology Academic Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands

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