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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 295, Issue 1, pp 99–102 | Cite as

Influence of viability on bromosulfophthalein uptake by isolated hepatocytes

  • M. Schwenk
  • R. Burr
  • E. Pfaff
Short Communication

Summary

The initial rates of BSP uptake by isolated hepatocytes were compared in cells of good and poor viability. Cells with impaired viability were obtained by ageing or by accident also in fresh preparations. Viability was judged by trypan blue stainability, membrane potential and respiratory parameters indicative for energy state, substrate supply and plasma membrane permeability changes. It was found that concomitant with impaired viability there was a decline of uptake rates at low and an increase at high BSP concentrations with a crossover point at 10 μM as manifest in an increase of K m and V. Simultaneously, the affinity and size of the membrane bound fraction decreases. The results give kinetic support to the supposition that it is the decreased uptake from plasma to liver that is responsible for the prolonged plasma retention times in the liver function test of patients with impaired hepatobiliary function.

Key words

Cell isolation Cell survival Liver cytology BSP uptake Cell membrane permeability 

Abbreviations

BSP

Bromosulfophthalein

CCP

Carbonylcyanide m-chlorophenylhydrazone

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References

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • M. Schwenk
    • 1
  • R. Burr
    • 1
  • E. Pfaff
    • 1
  1. 1.Institut für Toxikologie der Universität TübingenTübingenFederal Republic of Germany

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