Influence of viability on bromosulfophthalein uptake by isolated hepatocytes
The initial rates of BSP uptake by isolated hepatocytes were compared in cells of good and poor viability. Cells with impaired viability were obtained by ageing or by accident also in fresh preparations. Viability was judged by trypan blue stainability, membrane potential and respiratory parameters indicative for energy state, substrate supply and plasma membrane permeability changes. It was found that concomitant with impaired viability there was a decline of uptake rates at low and an increase at high BSP concentrations with a crossover point at 10 μM as manifest in an increase of K m and V. Simultaneously, the affinity and size of the membrane bound fraction decreases. The results give kinetic support to the supposition that it is the decreased uptake from plasma to liver that is responsible for the prolonged plasma retention times in the liver function test of patients with impaired hepatobiliary function.
Key wordsCell isolation Cell survival Liver cytology BSP uptake Cell membrane permeability
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- Baur, H., Kasperek, S., Pfaff, E.: Criteria of viability of isolated liver cells. Hoppe-Seylers Z. physiol. Chem. 356, 827–838 (1975)Google Scholar
- Deutsch, E.: A fractional bromsulphthalein test to determine liver damage in the non-jaundiced patient. New Engl. J. Med. 225, 171–175 (1941)Google Scholar
- Ingelfinger, F. J., Bradley, S. E., Mendeloff, A. I., Kramer, P.: Studies with bromsulphalein. 1. Its disappearance from blood after a single intravenous injection. Gastroenterology 11, 646–657 (1948)Google Scholar
- Jablonski, P., Owen, J. A.: Clinical chemistry of bromsulfophthalein and other cholephilic dyes. Advanc. clin. Chem. 12, 309–386 (1969)Google Scholar
- Javitt, N. B., Wheeler, H. O., Baker, K. J., Ramos, O. L., Bradley, S. E.: The intrahepatic conjugation of sulphobromophthalein and glutathione in the dog. J. clin. Invest. 39, 1570–1577 (1960)Google Scholar
- Pfaff, E., Schwenk, M., Burr, R., Remmer, H.: Molecular aspects of the interaction of bromosulfophthalein with high-affinity binding sites of bovine serum albumin. Molec. Pharmacol. 11, 144–152 (1975)Google Scholar
- Richards, T. G., Tindall, V. R., Young, A.: A modification of the bromsulphthalein liver function test to predict the dye content of the liver and bile. Clin. Sci. 18, 499–511 (1959)Google Scholar
- Schwarz, L. R., Burr, R., Schwenk, M., Pfaff, E., Greim, H.: Uptake of taurocholic acid into isolated rat-liver cells. Europ. J. Biochem. 55, 617–623 (1975)Google Scholar
- Schwenk, M., Burr, R., Schwarz, L., Pfaff, E.: Uptake of bromosulfophthalein by isolated liver cells. Europ. J. Biochem. 64, 189–197 (1976)Google Scholar