DNA synthesis in immunreactive glandular cells in pituitary intermediate lobe cultures
- 18 Downloads
Cell suspensions were prepared from adult rat pituitary intermediate lobes and grown in medium TC 199 supplemented with foetal calf serum. 7 or 8 days old cultures were pulse labelled with tritiated thymidine and thereafter processed for immunostaining using an antiserum against a synthetic α-1-28-ACTH-analogue.
Immunreactivity was mainly confined to epithelial cells, but some spindle-shaped cells were also immunpositive. Thymidine incorporation was observed in some immunopositive cells which shows that cells committed to hormone production may enter the mitotic cycle.
KeywordsPublic Health Epithelial Cell Cell Suspension Foetal Calf Serum Thymidine
Unable to display preview. Download preview PDF.
- Bácsy, E., Tougard, C., Tixier-Vidal, A., Marton, J., Stark, E.: Corticotroph cells in primary cultures of rat adenohypophysis: a light and electron microscopic immuno-cytochemical study. Histochemistry, 50, 161–174 (1976)Google Scholar
- Davies, C., Jacobi, J., Lloyd, H.M., Meares, J.: DNA synthesis and the secretion of prolactin and growth hormone by the pituitary gland of the female rat: effect of diethylstilboestrol and 2-bromo-α-ergocryptine methanesulphonate. J. Endocrinol. 61, 411–417 (1974)Google Scholar
- Griswold, M.D., Solari, A., Rung, S.P., Fritz, B.J.: Stimulation by follicle-stimulating hormone of DNA synthesis and of mitosis in cultured sertoli cells prepared from testes of immature rats. Mol. Cell. Endocrinol. 7, 151–165 (1977)Google Scholar
- Gyévai, A., Rappay, Gy., Kondies, L., Stark, E.: Hormone production and cell division of adrenal cells from adult rats in cell cultures. (In Hungarian). Biol. Közl. 21, 31–40 (1973)Google Scholar
- Leach, F.N., Ashworth, M.A., Barson, A.J., Milner, R.D.G.: Insulin release from human foetal pancreas in tissue culture. J. Endocrinol. 59, 65–79 (1973)Google Scholar
- Nakane, P.K.: Classification of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 (1970)Google Scholar
- Rappay, G., Gyévai, A., Kondics, L., Stark, E.: Growth and fine structure of monolayers derived from adult rat adenohypophyseal cell suspensions. In Vitro 8, 301–306 (1973)Google Scholar
- Rappay, Gy., Fazekas, I., Bácsy, E., Kárteszi, M.: Spontaneous ACTH release in pituitary intermediate-lobe cell cultures. In preparation (1977)Google Scholar
- Stratmann, I.E., Ezrin, C., Sellers, E.A., Simon, G.T.: The origin of thyreoidectomy cells as revealed by high resolution autoradiography. Endocrinology 90, 728–734 (1972)Google Scholar
- Tashjian, A.H., Jr.: Animal cell cultures as a source of hormones. Biotechn. Bioengin. 11, 109–126 (1969)Google Scholar
- Tashjian, A.H., Jr., Hoyt, R.F., Jr.: Transient controls of organ-specific functions in pituitary cells in culture. In: Molecular genetics and developmental Biology ed. Maurice Sussman, pp. 353–387. Englewood Cliffs, New Jersey: Prentice Hall Inc. 1972Google Scholar
- Tixier-Vidal, A.: Ultrastructure of anterior pituitary cells in culture. In: The anterior pituitary (eds. Tixier-Vidal and M.G. Farquhar), pp. 181–229. New York: Academic Press 1975Google Scholar
- Zamboni, L., DeMartino, C.: Buffered picric acid-formaldehyde: A new, rapid fixative for electron microscopy, J. Cell Biol. 35, 148A (1967)Google Scholar