Histochemistry

, Volume 83, Issue 5, pp 409–411 | Cite as

“Thiocyanate gold”: small (2–3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy

  • W. Baschong
  • J. M. Lucocq
  • J. Roth
Article

Summary

Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation ∼15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates.

By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing α-amylase in rat pancreatic acinar cells.

Keywords

Public Health Gold Electron Microscope Serial Dilution Thiocyanate 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • W. Baschong
    • 1
  • J. M. Lucocq
    • 2
  • J. Roth
    • 2
  1. 1.Department of MicrobiologyBiozentrum of the University of BaselBaselSwitzerland
  2. 2.Interdepartmental Electron MicroscopyBiozentrum of the University of BaselBaselSwitzerland

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