“Thiocyanate gold”: small (2–3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy
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Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation ∼15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates.
By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing α-amylase in rat pancreatic acinar cells.
KeywordsPublic Health Gold Electron Microscope Serial Dilution Thiocyanate
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