, Volume 83, Issue 5, pp 401–404 | Cite as

Immunocytochemical localization of aromatase in rat testis

  • M. Kurosumi
  • K. Ishimura
  • H. Fujita
  • Y. Osawa


The immunocytochemical localization of armatase in the testes of young and adult rats was investigated by an indirect-immunofluorescent method using antihuman placental aromatase-II cytochrome P-450 antibody. In both young (1 and 2 weeks old) and adult rats, only the Leydig cells in the interstitial tissue showed a positive immunoreaction for aromatase, while the germ cells and Sertoli cells in the seminiferous tubule were entirely negative. In addition, electron microscopy revealed that the Leydig cells in the testes of young as well as adult rats have a well-developed smooth endoplasmic reticulum, mitochondria with tubulovesicular cristae, and a few lipid droplets; these structures being characteristic of steroid secretory cells. On the basis of these results, we suggest that estrogens are mainly synthesized in Leydig cells of the testes.


Lipid Electron Microscopy Steroid Estrogen Endoplasmic Reticulum 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Kurosumi
    • 1
  • K. Ishimura
    • 1
  • H. Fujita
    • 1
  • Y. Osawa
    • 2
  1. 1.Department of AnatomyOsaka University Medical SchoolOsakaJapan
  2. 2.Medical Foundation of BuffaloBuffaloUSA

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