Summary
The ceramides and steryl-sulfate components from desquamated and cohesive human stratum corneum were examined using a combination of chemical and chromatographic means. Six structurally distinct series of ceramides were identified, and the relative amounts of these species, as measured by quantitative thin-layer chromatography, did not differ in cohesive and desquamated stratum corneum. In contrast, the level of cholesteryl sulfate was significantly reduced in the desquamated material. The results are in accord with the hypothesis that cholesteryl sulfate serves in cell-to-cell cohesion within the stratum corneum, and its hydrolysis may be necessary to permit shedding of cells from the surface.
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References
Breathnach AS, Goodman T, Stolinski C, Gross M (1973) Freeze fracture replication of cells of stratum corneum of human epidermis. J Anat 114:65–81
Downing DT (1968) Photodensitometry in the thin-layer chromatographic analysis of neutral lipids. J Chromatogr 38:91–99
Downing DT, Stranieri AM (1980) Correction for the deviation from the Lambert-Beer law in the quantitation of thin-layer chromatograms by photodensitometry. J Chromatogr 192:208–211
Elias PM, Brown BE (1978) The mammalian cutaneous permeability barrier. Defective barrier function in essential fatty acid deficiency correlates with abnormal intercellular lipid deposition. Lab Invest 39:574–583
Elias PM, Friend DS (1975) The permeability barrier in mammalian epidermis. J Cell Biol 65:180–191
Gray GM, White RJ (1978) Glycolipids and ceramides in human and pig epidermis. J Invest Dermatol 70:336–341
Gray GM, Yardley HJ (1975) Different populations of pig epidermal cells: isolation and lipid composition. J Lipid Res 16:441–447
Karlsson KA, Pascher I (1971) Thin-layer chromatography of ceramides. J Lipid Res 12:466–472
Matoltsy AG (1976) Keratinization. J Invest Dermatol 67:20–25
Shapiro LJ, Weiss R, Webster D, France JT (1978) Enzymatic basis of typical x-linked ichthyosis. Lancet 1:70–72
Smith WP, Christensen MS, Nacht S, Gans EH (1982) Effect of lipids on the aggregation and permeability of human stratum corneum. J Invest Dermatol 78:7–11
Squier CA (1973) The permeability of keratinized and nonkeratinized oral epithelium to horseradish peroxidase. J Ultrastruct Res 43:160–177
Wertz PW, Downing DT (1983) Ceramides of pig epidemis: structure determination. J Lipid Res 24:759–765
Wertz PW, Cho ES, Downing DT (1983) Effect of essential fatty acid deficiency on the epidermal sphingolipids of the rat. Biochim Biophys Acta 753:350–355
Wertz PW, Colton SW, Downing DT (1983) Comparison of the hydroxyacids from the epidermis and from the sebaceous glands of the horse. Comp Biochem Physiol [B] 75:217–220
Williams ML (1983) The ichthyoses — pathogenesis and prenatal diagnosis: a review of recent advances. Pediatr Dermatol 1:1–24
Williams ML, Elias PM (1981) Stratum corneum lipids in disorders of cornification. Increased cholesterol sulfate content of stratum corneum in recessive x-linked ichthyosis. J Clin Invest 68:1404–1410
Yardley HJ, Summerly R (1981) Lipid composition and metabolism in normal and diseased epidermis. Pharmacol Ther 13:357–383
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Long, S.A., Wertz, P.W., Strauss, J.S. et al. Human stratum corneum polar lipids and desquamation. Arch Dermatol Res 277, 284–287 (1985). https://doi.org/10.1007/BF00509081
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DOI: https://doi.org/10.1007/BF00509081