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Archives of Dermatological Research

, Volume 273, Issue 1–2, pp 129–135 | Cite as

Experimental acantholysis by complement-fixing intercellular antibodies

  • Takashi Hashimoto
  • Makoto Sugiura
  • Seiichi Kurihara
  • Takeji Nishikawa
  • Hitoshi Hatano
Original Contributions

Summary

Complement-fixing intercellular antibodies were detected in 10 of 17 sera from untreated pemphigus patients. The role of complement in the organ culture system was investigated using these sera. Ten sera possessing complement-fixing intercellular antibodies showed IgG binding to the intercellular substance in the organ-cultured skin and acantholysis-like changes were observed in eight cases. C3 deposition was not seen in any case. However, after treatment of the sections of cultured skin with fresh normal human serum, complement fixation of the intercellular substance by bound IgG was revealed in all the ten cases. No significant differences in the grade of acantholysis-like changes between the complement-depleted system and the complement-supplied system were observed. Complement does not appear to be necessary in the acantholytic process in the in vitro organ culture system, even though we considered the presence of complement-fixing intercellular antibodies.

Key words

Pemphigus Intercellular antibody Complement Organ culture 

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References

  1. 1.
    Cormane RH, Chorzelski TP (1967) Bound complement in the epidermis of patients with pemphigus vulgaris. Dermatologica 134:463–466Google Scholar
  2. 2.
    Cram DL, Fukuyama K (1972) Immunochemistry of ultraviolet-induced pemphigus and pemphigoid lesions. Arch Dermatol 106:819–826Google Scholar
  3. 3.
    van Joost T, Cormane RH, Pondman KW (1971) Direct immunofluorescence study of the skin on occurrence of complement in pemphigus. Br J Dermatol 81:466–474Google Scholar
  4. 4.
    Jordon RE, Schroeter AL, Rogers RS III, Perry HO (1974) Classical and alternate pathway activation of complement in pemphigus vulgaris lesions. J Invest Dermatol 63:256–259Google Scholar
  5. 5.
    Jordon RE, Sams WM Jr, Diaz G, Beutner EH (1971) Negative complement immunofluorescence in pemphigus. J Invest Dermatol 56:407–410Google Scholar
  6. 6.
    Nishikawa T, Kurihara S, Harada T, Sugawara M, Hatano H (1977) Capability of complement fixation of phemphigus antibodies in vitro. Arch Dermatol Res 260:1–6Google Scholar
  7. 7.
    Hashimoto T, Nishikawa T, Kurihara S, Hatano H (1978) Complement fixing pemphigus antibodies. Arch Dermatol 114:1191–1192Google Scholar
  8. 8.
    Kurihara S (1978) Studies of the complement fixing antibodies in bullous pemphigoid and pemphigus. Jpn J Dermatol 88:435–441 (In Japanese)Google Scholar
  9. 9.
    Schiltz J, Michel B (1976) Production of epidermal acantholysis in normal human skin in vitro by the IgG fraction from pemphigus serum. J Invest Dermatol 67:254–260Google Scholar
  10. 10.
    Deng JS, Beutner EH, Shu S, Chorzelski TP (1976) Pemphigus antibody action on skin explant. Kinetics of acantholytic changes and stability of antigens in tissue culture of normal monkey skin explants. Arch Dermatol 113:923–926Google Scholar
  11. 11.
    Binder WL, Beutner EH, Chorzelski TP (1978) In vitro effects of pemphigus antibodies on skin. Br J Dermatol 99:39–42Google Scholar
  12. 12.
    Barnett ML, Beutner EH, Chorzelski TP (1977) Organ culture studies of pemphigus. J Invest Dermatol 68:256–271Google Scholar
  13. 13.
    Hu C, Michel B, Schiltz JR (1978) Epidermal acantholysis induced in vitro by pemphigus autoantibody. Am J Pathol 90:345–351Google Scholar
  14. 14.
    Gschnait F, Pehamberger H, Holubar K (1978) Pemphigus acantholysis in tissue culture: Studies on photoinduction. Acta Derm Venereol (Stockh) 58:237–239Google Scholar
  15. 15.
    Koshiyama Y (1980) Organ culture studies of pemphigus antibodies. Jpn J Dermatol 90:589–597 (In Japanese)Google Scholar
  16. 16.
    Beutner EH, Chorzelski TP, Jordon RE (1970) Autosensitization in pemphigus and bullous pemphigoid. CC Thomas, Springfield, ILGoogle Scholar
  17. 17.
    Jordon RE, Sams WM Jr, Beutner EH (1969) Complement immunofluorescent staining in bullous pemphigoid. J Lab Clin Med 74:548–556Google Scholar
  18. 18.
    Sarkany I, Grice K, Caron GA (1965) Organ culture of human skin. Br J Dermatol 77:65–76Google Scholar
  19. 19.
    Nishikawa T, Kurihara S, Hatano H (1979) Comparison of in vivo and in vitro capability of complement fixation by pemphigus antibodies. Dermatologica 159:290–294Google Scholar
  20. 20.
    Pehamberger H, Gschnait F, Konrad K, Holubar K (1980) Bullous pemphigoid, herpes gestationis and linear dermatitis herpetiformis: Circulating anti-basement membrane zone antibodies: in vitro studies. J Invest Dermatol 74:105–108Google Scholar
  21. 21.
    Nishikawa T, Kurihara S, Harada T, Sugawara M, Hatano H (1978) Capability of complement fixation by in vivo bound antibodies in pemphigus skin lesions. Clin Exp Dermatol 3:57–59Google Scholar
  22. 22.
    Yamasaki Y (1981) Immunopathological study on pemphigus perilesional skin; comparative light and electron microscopic study of the localization in vivo bound IgG and C3. J Dermatol (Tokyo) 8:165–173Google Scholar
  23. 23.
    Jablonska S, Beutner EH, Michel B et al. (1975) Uses for immunofluorescence test of skin and sera: Utilization of immunofluorescence in the diagnosis of bullous disease, lupus erythematosus and certain other dermatoses. Arch Dermatol 111:371–381Google Scholar
  24. 24.
    Kumar V, Yarbrough C, Beutner EH (1980) Complement fixing intercellular antibodies in a case of cicatricial pemphigoid. Arch Dermatol 116:812–814Google Scholar
  25. 25.
    Kumar V, Beutner EH, Chorzelski TP, Jablonska S (1978) Letter to the editor of Nishikawa, Kurihara, Harada, Sugawara, and Hatano, “Capability of complement fixation of pemphigus antibodies in vitro”. Arch Dermatol Res 263:239Google Scholar
  26. 26.
    Krasny SA, Beutner EH (1980) Tissue culture studies of pemphigus and human and experimentally induced pemphigus like antibodies (Abstr). J Invest Dermatol 74:247Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Takashi Hashimoto
    • 1
  • Makoto Sugiura
    • 1
  • Seiichi Kurihara
    • 1
  • Takeji Nishikawa
    • 1
  • Hitoshi Hatano
    • 1
  1. 1.Department of DermatologyKeio University School of MedicineTokyoJapan

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